满药赤雹根总皂苷抗炎作用实验研究

目的:评价赤雹根总皂苷(TSRT)的抗炎活性.方法:二甲苯致小鼠耳肿胀实验,昆明种小鼠,随机分为TSRT240,120,60 mg·kg-1剂量组,Asp 200 mg·kg-1组及模型对照组,连续ig给药3 d.角叉菜胶致大鼠足跖肿胀实验,Wistar大鼠,随机分为TSRT 200,100,50 mg·kg-1剂量组,Asp 160 mg·kg-1组及模型对照组,连续ig给药3d.大鼠皮内色素渗出实验,分组及给药剂量同“大鼠足跖肿胀实验”,ig给药1次.小鼠棉球肉芽肿实验,分组及给药剂量同“小鼠耳肿胀实验”,连续ig给药7d.以耳肿胀度、足跖肿胀度、色素渗出量及肉芽肿质量为指标评价TSRT对急、慢性炎症的影响.结果:在小鼠耳肿胀实验中,TSRT 240,120,60 mg·kg-1剂量组小鼠耳肿胀度均明显低于模型对照组(P＜0.01)；在大鼠足跖肿胀实验中,致炎后1-4h,TSRT 200,100 mg·kg-1剂量组大鼠足趾肿度均明显低于模型对照组(P＜0.01),致炎后2～4h,TSRT 50 mg·kg-1剂量组大鼠足趾肿度均明显低于模型对照组(P＜0.05)；大鼠皮内色素渗出实验中,各剂量组皮内蓝斑的面积及色素渗出量均低于模型对照组(P＜0.05～P＜0.01)；大鼠棉球肉芽肿质量显著低于模型对照组(P＜0.05～P＜0.01).结论:赤雹根总皂苷对急慢性炎症有明显的抗炎作用.

Experiment Study on Anti-inflammatory Effect of Total Saponins from Thladiantha dubia Root

Objective:To investigate the anti-inflammatory effect of total saponins of the root from Thladiantha dubia Bunge(TSRT).Method: The experiment of xylene-induced ear swelling in mice,carrageenin-induced paw edema in rats,histamine-induced pigment oozing from skin vessel in rats and cotton pellet granuloma in mice were used to investigate the effect of TSRT on acute and chronic inflammation.Result: In mice ear swelling test,the ear swelling degree in all TSRT dose groups was lower than that in model group(P<0.01).In the test of carrageenin-induced paw edema in rats,at 1-4 h after carrageenin-induced inflammatory,paw edema degree in TSRT 200 mg·kg-1 and 100 mg·kg-1 groups were significantly lower than that in mode group(P<0.01),at 2-4 h after carrageenin-induced inflammatory,paw edema degree in TSRT 50 mg·kg-1 group was lower than that in model group(P<0.05).In the test of histamine-induced pigment oozing from skin vessel in rats,in all TSRT dose groups,the pigment infiltration area and pigment content were lower than that in model group(P<0.05-P<0.01).In the test of mice cotton pellet granuloma,weight of cotton pellet granuloma was lower than that in model group(P<0.05-P<0.01).Conclusion: TSRT has significent anti-inflammatory on acute and chronic inflammatory.