柴朴汤对支气管哮喘豚鼠支气管肺组织中Bcl-2表达的影响

目的:观察中药柴朴汤对哮喘豚鼠支气管肺组织中Bc1-2表达的影响.方法:40只健康豚鼠随机分为正常对照组,哮喘模型组,地塞米松组(0.001 g·kg-1),柴朴汤组(0.35 g·kg-1),每组10只.采用卵蛋白腹腔注射和雾化吸入激发建立哮喘模型,各组豚鼠均于激发前30 min予相应灌胃处理,隔日1次,共7次.末次激发24h后,处死各组豚鼠.检测各组豚鼠血液中嗜酸性粒细胞数及支气管肺泡灌洗液(BALF)中白细胞(WBC)数,嗜酸粒细胞(EOS)数及百分比；HE染色法观察各组豚鼠支气管肺组织病理改变；免疫组化法检测支气管肺组织中Bcl-2蛋白表达,原位杂交法检测支气管肺组织中Bcl-2 mRNA的表达.结果:柴朴汤组外周血中EOS数,BALF中WBC敷、EOS数及百分比均较哮喘模型组明显降低(P＜0.05).与哮喘模型组比较,柴朴汤组支气管肺组织中Bcl-2 mRNA及蛋白的表达有显著性差异(P＜0.05).地塞米松组与柴朴汤组比较,无明显差异.结论:柴朴汤可以明显降低哮喘豚鼠外周血和支气管肺泡灌洗液中EOS,减轻支气管肺组织的炎症反应,下调Bcl-2 mRNA和蛋白的表达水平.

Effect of Chaipu Decoction on Expression of Bcl-2 in Lung Tissue in Asthma Guinea Pigs

Objective: To observe the effect of Chaipu decoction on expression of Bcl-2 in lung tissue in asthma guinea pigs. Method: Forty guinea pigs were randomized into normal control, model, dexamethasone (0.001 g·kg^-1) and chaipu decoction (0.35 g·kg^-1) groups with 10 guinea pigs each group. Asthma mouse model was prepared by injecting ovalbumin (OVA) into the abdominal cavity and inhalation of aerosol, and normal group, was given saline water. Each group was given relevant medicines by oral administration before 30 mins of suscitation corresponding, once every other day, for 2 weeks. All animals were sacrificed after the last stimulation. The white blood cell(WBC) count,the number of eosinophil(EOS) and the percentage of EOS in bronchoalveolar lavage fluid (BALF) of guinea pigs and the number of EOS in whole blood were examined. Pulmonary pathological changes were observed by HE stain. Bcl-2 protein expression was examined by immunohistochemistry. And Bcl-2 mRNA expression was detected with in situ hybridization. Result: Compared with model group, chaipu decoction group could significantly reduce the EOS count in whole blood, the WBC count,the number of EOS and the percentage of EOS in BALF(P<0.05); and the expression level of Bcl-2 mRNA and protein in the lung tissue were significantly decreased(P<0.05). In Chaipu decoction group, compared with dexamethasone group, the significant difference was not found. Conclusion: Chaipu decoction for asthma was able to reduce the proportion of EOS in BALF, alleviate the inflammation of airway and lung tissue of the asthmatic guinea pigs. Chaipu decoction for asthma could reduce the Bcl-2 mRNA and protein expression level.