枳实薤白桂枝汤对高脂血症大鼠血脂及血管内皮功能的影响

目的:观察枳实薤白桂枝汤对高脂血症大鼠血脂及血管内皮功能的影响.方法:以高脂饲料喂养建立高脂血症大鼠模型；分正常、模型、西药组、不同剂量中药干预组,共6组；西药组给予普伐他汀钠(3.6 mg·kg-1)灌胃,中药干预分别给予高(7.2 g·kg-1)、中(3.6 g·kg-1)、低剂量(1.8 g·kg-1)枳实薤白桂枝汤灌胃3周；测定总胆固醇(TC)、甘油三脂(TG)、高密度脂蛋白( HDL-c)、低密度脂蛋白(LDL-c)；一氧化氮(NO)、内皮素(ET-1)的含量.结果:与正常组比较,模型组大鼠血清TC,TG,LDL-C均显著升高(P＜0.01),HDL-C降低(P＜0.05)；血清NO水平降低(P＜0.05),血浆ET-1水平升高(P＜0.05).与模型组相比,普伐他汀钠组、枳实薤白桂枝汤高剂量组TC,TG,LDL-C均显著降低(P＜0.01),枳实薤白桂枝汤中、低剂量组TC,LDL-C均降低(P＜0.05),中剂量组TG降低(P＜0.05)；用药各组对HDL-C均无明显影响.普伐他汀钠组、枳实薤白桂枝汤高剂量组NO明显升高(P＜0.05),普伐他汀钠组ET-1明显降低(P＜0.05).结论:枳实薤白桂枝汤可明显降低高脂血症大鼠TC,TG和LDL-C含量,提高血清NO水平,对ET-1的释放没有明显影响.枳实薤白桂枝汤具有降血脂及部分改善血管内皮功能的作用.

The Effect of Zhishi Xiebai Guizhi Decoction on Lipid Metabolism and Vscular Endothelium in Hyperlipidemia Rat

Objective: To investigate the effects of Zhishi Xiebai Guizhi decoction on lipid metabolism and vscular endothelium in hyperlipidemia rat. Method: Using hyperlipidemia animal model replicated by hyperlipidemia forage, pravastatin sodium (3.6 mg·kg^-1)and different doses of Zhishi Xiebai Guizhi decocotion (7.2, 3.6, 1.8 g·kg^-1) were given to the model rats. Then the levels of total cholesterol(TC), triglycerides(TG), high-density liporotein cholesterol(HDL-C), low-density lipoprotein cholesterol(LDL-C) and levels of nitric oxide(NO), endothelin-1(ET-1) were assayed. Result: Compared with the normal group, the levels of TC,TG,LDL-C in the model group were increased obviously, the level of NO was decreased, the level of ET-1 was increased.Compared with the model group, the levels of TC,TG,LDL-C in the Zhishi Xiebai Guizhi decocotion high dose group and the pravastatin sodium group were decreased obviously, the level of NO was increased obviously, the level of ET-1 were decreased obviously. Conclusion: The Zhishi Xiebai Guizhi decocotion can regulate the lipid metabolism and improve vascular endothelium function in rat with hyperlipidemia.