血栓通对顺铂肾损伤大鼠的肾功能和氧化指标的影响

目的:研究血栓通对顺铂肾损伤大鼠肾功能的保护作用和其对损伤大鼠氧化指标的影响.方法:采用顺铂(0.5 mg·kg-1)尾静脉注射的方法制造顺铂肾毒性模型.将72只大鼠随机分为6组,空白组、模型组、安磷汀阳性药物组、血栓通低剂、中、高剂量组(15.63,31.35,62.70 mg·kg-1).分别给药处理10d后,观察大鼠24 h尿蛋白量、尿N-乙酰β-D氨基葡萄糖苷酶NAG,血清中肌酐、尿素氮,肾组织匀浆中超氧化物歧化酶(SOD)活性、谷胱甘肽过氧化物酶(GSH-Px)活性、丙二醛(MAD)含量及肾组织的病理变化.结果:与模型组比较,各治疗组大鼠血肌酐、血清尿素氮,24h尿蛋白量、尿NAG的含量明显降低,肾组织匀浆中SOD活性、GSH-Px活性明显增高,MAD含量明显降低(P ＜0.01,P＜0.05)；肾脏的病理变化明显减轻.结论:血栓通能有效改善顺铂肾损伤大鼠肾功能,降低肾组织氧化水平,减轻肾脏的病理变化,对大鼠顺铂肾损伤有保护作用.

Effect of Xueshuantong on Renal Function and Oxidation Indexes in Cisplatin-induced Nephroxicity Rats

Objective: To study the protective effect of Xueshuantong against cisplatin-induced nephrotoxicity. Method: Female SD rats were randomly divided into six groups:normal saline(NS)group,model group, positive control group and the high dose group, middle dose group and low dose group of Xueshuantong, with 12 rats in each group. The model rats with nephrotoxicity were duplicated with injection of cisplatin by vena caudalis. Rats in model group, positive control group and the high dose group, middle dose group and low dose group of Xueshuantong were treated by amifostine,0.1 mg·kg^-1, Xueshuantong, 15.63, 31.35, 62.70 mg·kg^-1 once a day.The changes of serum creatinine(SCr),blood urea nitrogen(BUN),N-acetyl-beta-D glucosa minidase(NAG),urine protein/24 h,the content of malondialdehyde(MDA) and the activity of superoxide dismutase(SOD),glutathione peroxidase(GSH-Px) were measured and renal structure was observed after 10 days of administration. Result: Compared with the model group, contents of BUN,SCr in serum and urine protein /24hour, NAG in urine were significantly decreased in each treatment group (P<0.01 or P<0.05), the content of MDA was also significantly decreased(P<0.01 or P<0.05)and the activity of SOD and GSH-Px was significantly increased (P<0.01 or P<0.05). The pathological slice indicated that renal structure was significantly improved. Conclusion: Xueshuantong can effectively relieve cisplatin-induced nephrotoxicity by reducing the pathological changes of renal structure and improving the function in renal injury rats induced by cisplatin and inhibiting the oxidative damage of kidney.