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奶粉中混合菌种检验方法的比较研究
引用本文:栾玲,李桂荣,伍海燕,王彦青,田云龙,韩文清. 奶粉中混合菌种检验方法的比较研究[J]. 中国病原生物学杂志, 2011, 0(12)
作者姓名:栾玲  李桂荣  伍海燕  王彦青  田云龙  韩文清
作者单位:烟台市疾病预防控制中心;
摘    要:目的研究奶粉中混合菌种的检测方法。方法用直接平板分离法和增菌培养法对奶粉中质控菌株进行增菌、分纯,依据细菌生长特性、菌落平板特征、涂片革兰染色镜检结果、生化试验和血清学鉴定结果综合判断。结果奶粉中质控菌株为混合菌种,分别是金黄葡萄球菌、大肠埃希菌O157∶H7。结论做混合菌种样品检测时,平板直接分离法效果较好;BHI肉汤培养诱导鞭毛抗原的方法值得推广使用。

关 键 词:混合菌种  菌种鉴定  奶粉  

Study comparing methods of testing milk powder for bacteria species
LUAN Ling,LI Gui-rong,WU Hai-yan,WANG Yan-qing,TIAN Yun-long,HAN Wen-qing. Study comparing methods of testing milk powder for bacteria species[J]. Journal of Pathogen Biology, 2011, 0(12)
Authors:LUAN Ling  LI Gui-rong  WU Hai-yan  WANG Yan-qing  TIAN Yun-long  HAN Wen-qing
Affiliation:LUAN Ling,LI Gui-rong,WU Hai-yan,WANG Yan-qing,TIAN Yun-long,HAN Wen-qing (Yantai Center for Disease Control and Prevention,Yantai 264003,Shandong,China)
Abstract:Objective To study methods of detecting bacteria species in milk powder. Methods Enrichment culture and direct plate isolation were used to grow and isolate species of bacteria controlled during milk powder production.A comprehensive determination was made based on species growth characteristics,colony characteristics on plates,microscopic examination of Gram strains,and results of biochemistry and serology tests. Results Multiple species were controlled during milk powder production,including Staphylococcu...
Keywords:Bacteria species  species identification  milk powder  
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