四物合剂对化疗后大鼠卵巢形态及颗粒细胞凋亡的影响

目的:探讨四物合剂对化疗所致卵巢储备功能下降的大鼠卵巢颗粒细胞凋亡的影响.方法:以40只雌性SD大鼠为研究对象,随机分为正常对照组(正常组)、顺铂模型组(模型组)、四物合剂组(四物组)和倍美力组,每组各10只.模型组、四物组和倍美力组连续7d腹腔注射顺铂2mg·kg-1·d-1,正常组连续7d腹腔注射等量的生理盐水,随后正常组和模型组连续7d纯净水灌胃10 mL·kg-1·d-1,四物组连续7d四物合剂灌胃1mg·kg-1·d-1、倍美力组连续7d倍美力溶液灌胃1 mg·kg-1·d-1.自实验第1天起,每日制作大鼠阴道分泌物涂片以观察大鼠动情周期；实验第14天大鼠称重、麻醉,取血清运用放免法测血中卵泡刺激素( FSH)、黄体生成激素(LH)、雌二醇(E2)和孕酮(Pro):取双侧卵巢,同侧切片后常规HE染色,图像分析仪下进行颗粒细胞层最大厚度的显微测量,并运用原位细胞死亡检测法检测颗粒细胞凋亡情况.结果:与正常组比较,模型组大鼠FSH和凋亡指数均升高,差异有统计学意义(P＜0.05),同时E2值下降.与模型组比较,四物组大鼠FSH和凋亡指数降低,差异有统计学意义(P＜0 05),同时E2值升高.结论:四物合剂对化疗后大鼠卵巢颗粒细胞的凋亡有一定的抑制作用,能促进卵巢功能恢复,但还不能起到完全修复的作用.

The Effects of Siwu Mixture on Ovarian Granulose Cell Apoptosis of Chemotherapy-Induced Rats

Objective: To explore the effects of Siwu mixture on chemotherapy-induced decline in ovarian reserve function of rat and rat's ovarian granulosa cell apoptosis. Method: Forty female SD rats randomly divided into normal control group, Cisplatin model group, Siwu treatment groups and Premarin treatment group.Each set was 10 female SD rats. Model group, Siwu group and Premarin group were consecutive intraperitoneal injected of cisplatin (2 mg·kg^-1·d^-1)for 7 days. The normal group was consecutive intraperitoneal injected of the same amount of normal saline for 7 days. Then the normal group and model group were continuous intragastric infusion with pure watergroups(10 mL·kg^-1·d^-1) for 7 days, Siwu group was continuous intragastric infusion with Siwu mixture(10 mL·kg^-1·d^-1) for 7 days, and Premarin group was continuous intragastric infusion with premarin(10 mL·kg^-1·d^-1) for 7 days. Since the first day up, we made vaginal secretion real smear to observe rat's estrous cycle daily. At the experimental 14 day, we weighed and gave anesthesia to rats, to take serum of rats and using radio-immunity method measuring blood follicle-stimulating hormone(FSH), luteotrophic hormone(LH), estradiol(E₂) and progestins(Pro). We took double side ovarian of rats,with the same side slice for HE dyeing, and imaged micro measurement the particles cell layer maximum thickness at Image Analyzer, then using TUNEL method to detected particles cell apoptosis. Result: Compared with the normal group, the FSH and apoptosis index were elevated in model group rats,the difference statistically significant (P<0.05), while E₂ is decreased. Compared with the model group, FSH and apoptosis index in Siwu group rats were lower,the difference statistically significant (P<0.05), while the E₂ values were rise. Conclusion: Siwu mixture has a certain degree of inhibition effects on ovarian granulosa cell apoptosis in rat after chemotherapy, but cannot afford to completely fix it.