| 乙型脑炎病毒分型基因芯片的制备 |
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| 引用本文： | 郭欢欢,凡敏,鲁会军,刘振江,齐延新,秦艳青,岳云强,袁森,崔鹤馨,田宇飞,刘昊,田明尧,金宁一.乙型脑炎病毒分型基因芯片的制备[J].中国病原生物学杂志,2011(11). |
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| 作者姓名： | 郭欢欢 凡敏 鲁会军 刘振江 齐延新 秦艳青 岳云强 袁森 崔鹤馨 田宇飞 刘昊 田明尧 金宁一 |
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| 作者单位： | 吉林农业大学动物科技学院;军事医学科学院军事兽医研究所;吉林大学畜牧兽医学院; |
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| 基金项目： | 国家课题支撑计划项目(No.2010BAD04B02) |
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| 摘 要： | 目的制备乙型脑炎病毒分型基因芯片。方法根据乙型脑炎病毒的基因组学序列,应用生物学软件设计乙型脑炎病毒分型引物及探针,制备JEV分型基因芯片。PCR扩增荧光标记的片段与固定于玻片上的探针杂交后,进行芯片清洗、扫描及结果分析。通过特异性、灵敏性、重复性试验验证,建立分型基因芯片检测方法。结果杂交显示,设计的6条探针中有3条特异地与相应的标记样品杂交并在芯片上呈现较强的阳性杂交信号,其中1号探针能特异性检出乙型脑炎病毒,4、5号探针可对JEV-Ⅰ和JEV-Ⅲ型进行分型。制备的基因芯片具有可靠的重复性。结论建立的基因芯片具有高特异性、灵敏性及良好的重复性,可以快速、准确、高通量地对乙型脑炎病毒进行分型检测。
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| 关 键 词： | 乙型脑炎病毒 基因型 基因芯片 |
Development of a gene chip to detect subtypes of Japanese encephalitis virus |
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| GUO Huan-huan,FAN Min,LU Hui-jun,LIU Zhen-jiang,QI Yan-xin,QIN Yan-qing,YUE Yun-qiang,YUAN Sen,CUI He-xin,TIAN Yu-fei,LIU Hao,TIAN Ming-yao,JIN Ning-yi.Development of a gene chip to detect subtypes of Japanese encephalitis virus[J].Journal of Pathogen Biology,2011(11). |
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| Authors: | GUO Huan-huan FAN Min LU Hui-jun LIU Zhen-jiang QI Yan-xin QIN Yan-qing YUE Yun-qiang YUAN Sen CUI He-xin TIAN Yu-fei LIU Hao TIAN Ming-yao JIN Ning-yi |
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| Institution: | GUO Huan-huan1,2,FAN Min1,LU Hui-jun2,LIU Zhen-jiang1,QI Yan-xin2,3,QIN Yan-qing2,YUE Yun-qiang1,YUAN Sen1,CUI He-xin2,TIAN Yu-fei2,LIU Hao2,TIAN Ming-yao2,JIN Ning-yi2 (1.College of Animal Science and Technology,Jilin Agricultural University,Changchun 130118,China,2.Institute of Military Veterinary,PLA Academy of Military Medical Sciences,Changchun 130122,3.College of Animal Science and Veterinary Medicine,Jilin University,Changchun 130062,China) |
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| Abstract: | Objective To fabricate a gene chip to detect subtypes of the Japanese encephalitis virus.Methods Probes and primers were designed with bioinformatics software based on the sequence of the JEV genome to detect subtypes of the JEV and fabricate oligonucleotide microarrays.Amplified by PCR,fluorescently labeled fragments were hybridized with oligonucleotide microarrays and then washed,scanned,and analyzed.A method of detecting subtypes of the JEV was established through specificity,sensitivity,and repeatabilit... |
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| Keywords: | Japanese encephalitis virus genotype gene chip |
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