飞龙掌血乙醇提取物对白色念珠菌抑菌作用的研究

目的:研究飞龙掌血乙醇提取物体外抑制白色念珠菌生长作用,为进一步开发根管消毒新药提供理论依据.方法:微量肉汤稀释法确定飞龙掌血乙醇提取物体外抑制白色念珠菌生长的最小抑菌浓度(MIC)及最小杀菌浓度(MBC)；常规扫描电镜制样,观察细菌细胞形态的变化；采用RT-PCR技术获得互补DNA,PCR技术扩增白色念珠菌毒力因子SNF2和PDE2基因片段,产物进行电泳分析.结果:飞龙掌血乙醇提取物对白色念珠菌的最小抑菌浓度为7.5 g·L-1,最小杀菌浓度为15.0 g·L-1；扫描电镜观察药物作用后菌体呈现不规则形态,表面有明显的凹陷、裂痕；随着药物浓度增加,毒力因子SNF2和PDE2的表达量呈明显下降趋势.结论:飞龙掌血乙醇提取物对白色念珠菌有较好的抑菌活性；推断该药物是通过抑制或干扰毒力因子SNF2和PDE2转录过程的表达,从而抑制致病性菌丝形态的形成及破坏细菌细胞壁的完整性,达到抑菌作用.

The Antibacterial Activity of the Ethanol Extract Toddalia asiatica against Candida albicans

Objective: To study the inhibition action of ethanol extract Toddalia asiatica in vitro against candida albicans growth and provide the theory basis for new drugs for the further development of the root canal disinfection. Method: Trace broth dilution method was used to determine the minimum inhibition concentration (MIC) and minimum bactericidal concentration (MBC) of the ethanol extract T. asiatica in vitro. Conventional scanning electron microscopy sample preparation was used to observe change of bacterial cells. The RT-PCR technology used to obtain complementary DNA, PCR amplification technology used to obtain SNF2 and PDE2 gene fragments of C.albicans virulence factors, products electrophoresis analysis was performed. Result: The MIC was 7.5 g·L^-1, MBC was 15.0 g·L^-1; scanning electron microscopy showed a irregular morphological changes of bacteria, the surface had obvious sag, crack; with the increased drug concentration, SNF2 and PDE2 expression was obviously declined. Conclusion: The ethanol extract T. asiatica on C. albicans has a good antibacterial action, which may be related to inhibiting expression of SNF2 and PDE2.