参术胶囊对脾虚胃癌小鼠ERK信号通路中AP-1和IL-2的影响

目的:研究参术胶囊对脾虚胃癌小鼠细胞外调节蛋白激酶(ERK)信号通路中转录激活蛋白-1(AP-1)和白细胞介素-2(IL-2)的影响,以探讨参术胶囊阻断脾虚胃癌发生的机制.方法:随机取17只SPF小鼠作为空白组正常喂养,其余小鼠均首日给予山西白醋15 mL·kg-1·d-1灌胃,次日给予10 mL·kg-1·d-1灌胃,连续9d,第10天给予N-亚硝基二乙胺(DENA)按2.8 mg· kg-1·d-1灌胃,连续110 d,建立脾虚胃癌小鼠模型.将模型动物随机分为模型组、胃复春片718 mg·kg-1·d-1剂量组、参术胶囊440,220,110 mg·kg-1 ·d-1剂量组,试验组从111 d起灌胃给药,连续30 d.实验结束后,观察各组小鼠一般情况的变化;HE染色观察参术胶囊是否阻断脾虚胃癌的发生;用免疫组化法研究参术胶囊对诱导脾虚胃癌模型ERK1/2,AP-1,IL-2等指标的影响.结果:参术胶囊作用脾虚胃癌小鼠模型30 d后,能改善诱发型脾虚胃癌小鼠模型一般状态,其病理表现较模型组明显减轻,免疫组化结果显示胃复春片组和参术胶囊440,220 mg·kg-1 ·d-1剂量组均能明显增强模型动物ERK1/2,AP-1,IL-2的表达(P ＜0.05,P ＜0.01),参术胶囊110 mg·kg-1·d-1剂量组能明显增强模型动物ERK1/2的表达(P＜0.01),而对AP-1,IL-2的表达无明显改善.结论:参术胶囊可通过ERK信号通路阻断脾虚胃癌发生.

The Effect of Shenzhu Capsules on AP-1 and IL-2 of ERK Signal Pathway in Gastric Cancer Mice with Spleen-deficiency

Objective: To study the effect of Shenzhu capsules on AP-1 and IL-2 of ERK signal pathway in the gastric cancer mice with spleen-deficiency and explore the molecular mechanism of Shenzhu Caspules in inhibiting the generation of gastric cancer mice with spleen-deficiency. Method: Seventeen mice were randomly selected as normal group. The other mice were administrated by 15 mL·kg^-1·d^-1Shanxi white venigar on the first day and 10 mL·kg^-1·d^-1from the second day for continuous 9 days and by 2.8 mg·kg^-1·d^-1 diethylnitrosamine(DENA) for 110 days to establish gastric cancer mice model with spleen-deficiency.The model mice were divided into model group, Weifuchun group, and 3 doses of Shenzhu capsules groups(440, 220 and 110 mg·kg^-1·d^-1). The mice of treatmental groups were administracted on the 111^th day and for continuous 30 days. The general state of animals were observed,the HE staining was used to study whether Shenzhu capsules inhibited the occurance of gastric cancer with spleen-deficiency and the immunohistochemistry method was used to study the influence of Shenzhu capsules on the ERK1/2,AP-1 and IL-2 expression when the experiment was over. Result: The general state and pathological manifestation of Shenzhu capsule groups mice was improved obviously compared with that of model group ones. The Weifuchun and the 440, 220 mg·kg^-1·d^-1Shenzhu capsules could obviously strengthen expression of ERK1/2,AP-1 and IL-2(P<0.05,P<0.01).110 mg·kg^-1·d^-1Shenzhu capsules could obviously strengthen the expression of ERK1/2(P<0.01) but not obviously strengthen the expression of AP-1 and IL-2. Conclusion: Shenzhu capsules can inhibit the occurance of gastric cancer with spleen-deficiency by inactivating ERK signal pathway.