复方青秦对痛风性肾病大鼠肾组织病理学的影响

目的: 探讨复方青秦对痛风性肾病大鼠肾组织病理学改变的影响。 方法: 将禁食16 h的雄性SD大鼠随机分为对照组和造模组,采用腺嘌呤诱导痛风模型,造模18 d,确认造模成功后,将造模组随机分为模型组、别嘌醇组、复方青秦377,188.50,94.25 mg·kg^-1组,每组各5只。各组灌胃给予相应药物6周,于末次药后16 h处死,摘取肾脏组织置于10%中性福尔马林溶液固定,经包埋、系列切片,进行HE和PAS染色,采用光镜对肾组织病变进行分级评分统计,并在PAS染色下测定肾小球系膜基质相对面积。 结果: 复方青秦377 mg·kg^-1组的肾小管上皮细胞变性坏死及尿酸盐结晶病变较模型组明显减轻(P<0.05),而其肾小球基质相对面积与模型组比较未见明显差异。 结论: 复方青秦377 mg·kg^-1给大鼠ig 6周,能明显减轻痛风大鼠肾组织病变,对肾脏组织具有保护作用。

Pathology effect of Compound Qingqin on Kidneys of Rats with Gouty Nephropathy

Objective: To investigated the pathology effect of compound Qingqin on kidneys of rats with gouty nephropathy. Method: Male SD rats were randomly divided into 2 groups:normal group and model group. The model rats were induced by adenine. After the model was determined successfully, all model rats were divided into model group, allopurinol group, compound Qingqin 377 mg·kg^-1group, Qingqin 188.50 mg·kg^-1 group and Qingqin 94.25 mg·kg^-1group, each consisting of 5 animals. Each group was given the corresponding drugs for 6 weeks. All rats were killed at 16 h after last dosing, then kidneys were taken out and fixed immediately with 10% formalin, embedded with paraffin, serially sectioned and stained with hematoxylin eosin (H.E) and periodic acid-sliver (PAS). To be classified and scored the renal lesions under the light microscope and the relative area of mesangium matrix was measared by under the PAS dyeing. Result: The renal tubular cells degeneration necrosis and uric-acid salts crystals lesions in the compound Qingqin 377 mg·kg^-1 group was significantly lightened than the model group (P<0.05), while its mesangium matrix relative area was no obvious difference from the model group. Conclusion :The compound Qingqin can significantly lighten the renal lesions of rats with gouty nephropathy after it is taken for 6 weeks by 377 mg·kg^-1 and it has a protective effect to kidney.