| 犬细粒棘球绦虫感染LAMP检出日期的确定 |
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| 引用本文： | 陈璐,吾拉木·马木提,陈洁,古力帕丽·麦曼提依明.犬细粒棘球绦虫感染LAMP检出日期的确定[J].中国病原生物学杂志,2011(11). |
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| 作者姓名： | 陈璐 吾拉木·马木提 陈洁 古力帕丽·麦曼提依明 |
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| 作者单位： | 新疆医科大学基础医学院自治区病原生物学重点实验室;新疆医科大学第一附属医院包虫病重点实验室; |
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| 基金项目： | 国家自然科学基金项目(No.81160202); 新疆医科大学研究生创新基金项目(No.MC2010-4) |
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| 摘 要： | 目的确定犬细粒棘球绦虫感染环介导等温扩增技术(LAMP)最早检出日期。方法家犬8只,口服喂饲细粒棘球绦虫原头节,建立细粒棘球绦虫感染动物模型。感染后40d收集犬粪,用LAMP检测粪便中细粒棘球绦虫目的基因并与传统的PCR比较。结果 8只犬均获得细粒棘球绦虫感染,感染后第18dLAMP法检查犬粪中细粒棘球绦虫目的基因均阳性,较普通PCR法提早2d。结论 LAMP法简便、快捷、灵敏,有望成为早期诊断犬细粒棘球绦虫感染的重要手段。
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| 关 键 词： | 细粒棘球绦虫 环介导等温扩增 cox1基因 检测 |
Determination of the date of LAMP diagnosis of canine Echinococcus granulosus infection |
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| CHEN Lu ,MAMUTI Wulamu,CHEN Jie,MAIMAITIYIMING Gulipali.Determination of the date of LAMP diagnosis of canine Echinococcus granulosus infection[J].Journal of Pathogen Biology,2011(11). |
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| Authors: | CHEN Lu MAMUTI Wulamu CHEN Jie MAIMAITIYIMING Gulipali |
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| Institution: | CHEN Lu 1,2,MAMUTI Wulamu1,CHEN Jie1,MAIMAITIYIMING Gulipali1,2 (1.Department of Parasitology,Basic Medical College,Xinjiang Medical University,Urumqi 830011,China,2.Hydatid Disease Key Laboratory,the First Affiliated Hospital,Xinjiang Medical University) |
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| Abstract: | Objective To determine the earliest point for LAMP detection of infection in dogs infected with Echinococcus granulosus.Methods Eight dogs were artificially infected with E.granulosus by oral intake of live protoscoleces.Feces were collected for 40 days post-infection,and target gene fragments were detected using LAMP and compared to results using conventional PCR.Results All eight dogs developed severe infections.LAMP detected target genes in dog feces on day 18 post-infection,which is 2 days earlier than ... |
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| Keywords: | Echinococcus granulosus LAMP cox1 detection |
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