| 弓形虫表面抗原SAG1的可溶性表达及其免疫学活性分析 |
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| 引用本文： | 朱思梅,周亚萍,朱荫昌,曹利民. 弓形虫表面抗原SAG1的可溶性表达及其免疫学活性分析[J]. 中国病原生物学杂志, 2011, 0(11) |
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| 作者姓名： | 朱思梅 周亚萍 朱荫昌 曹利民 |
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| 作者单位： | 常州21世纪生物技术研究所有限公司;江苏省血吸虫病/寄生虫病防治研究所; |
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| 摘 要： | 目的表达和纯化弓形虫表面抗原1(SAG1),并分析其免疫学活性。方法构建pET15b-SAG1质粒,转化到宿主菌E.coil BL21(DE3)中,经IPTG诱导表达,使用Ni 2+亲和层析柱对重组蛋白进行纯化,用间接ELISA检测目的蛋白的抗原性。结果 SDS-PAGE显示表达的SAG1蛋白为可溶性的,经纯化后其纯度可达到90%以上;ELISA检测表达蛋白能被弓形虫感染者血清识别;棋盘滴定显示重组SAG1蛋白适宜包被浓度为0.5μg/ml。结论本研究表达的SAG1蛋白为可溶性的,且具有良好的抗原性,为进一步建立弓形虫血清学诊断方法和研制弓形虫病疫苗奠定了基础。
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| 关 键 词： | 弓形虫 SAG1蛋白 蛋白表达 纯化 抗原性 |
Expression and analysis of the immunological activity of Toxoplasma gondii surface antigen SAG1 |
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| ZHU Si-mei,ZHOU Ya-ping,ZHU Yin-chang,,CAO Li-min. Expression and analysis of the immunological activity of Toxoplasma gondii surface antigen SAG1[J]. Journal of Pathogen Biology, 2011, 0(11) |
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| Authors: | ZHU Si-mei ZHOU Ya-ping ZHU Yin-chang CAO Li-min |
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| Affiliation: | ZHU Si-mei1,ZHOU Ya-ping1,ZHU Yin-chang1,2,CAO Li-min1 (1.Changzhou 21st Century Biotech Research Institute,Changzhou 213164,Jiangsu,China,2.Jiangsu Institute of Parasitic Diseases and Schistosomiasis) |
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| Abstract: | Objective To clone and express Toxoplasma gondii surface antigen1(SAG 1) and to analyze the immunological activity of the expressed protein SAG1. Methods The recombinant plasmid pET15b-SAG1 was constructed and then was transformed to Escherichia coil BL21(DE3) for expression under induction of IPTG.The recombinant protein was purified by metal-chelating affinity chromatography using Ni2+.ELISA was used to analyze the antigenicity of the recombinant antigen protein.Results SDS-PAGE indicated that the fusion ... |
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| Keywords: | Toxoplasma SAG1 protein protein expression protein purification antigenicity |
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