急性髓系白血病细胞p16基因结构及转录水平表达

目的：了解ｐ１６基因在急性髓系白血病（ＡＭＬ）发病中的意义。方法：用聚合酶链反应－单链ＤＮＡ构象多态性、Ｎｏｒｔｈｅｒｎｂｌｏｔ方法分析１６例ＡＭＬ细胞ｐ１６基因结构及转录水平表达。结果：１６例均无ｐ１６基因的缺失、突变。初治和复发的１２例ＡＭＬ的ｐ１６ｍＲＮＡ表达水平明显低于４例长期缓解者及正常对照。１例ｐ５３基因突变ＡＭＬ的ｐ１６ｍＲＮＡ有明显升高。结论：ｐ１６基因在ＡＭＬ发病中可能不占重要地位，而在ＡＭＬ病态造血的维持中起一定作用，在ｐ５３突变时其表达反馈性升高，显示一种反馈环路失衡，提示在研究单个基因的基础上观察细胞周期检测点功能具有重要意义。

The structure and mRNA expression of p16 gene in AML cells

OBJECTIVE: To explore the role of p16 gene in the pathogenesis of AML. METHODS: PCR-SSCP and Northern blot were used to analyse the structure and mRNA expression of p16 gene in 16 cases of AML. RESULTS: No deletion or mutation of p16 gene were found in all of the 16 cases. The p16 mRNA expression in 12 AML at diagnosis or relapse was significantly lower than that in 4 long term remission cases and normal controls. p16 mRNA expression was significantly higher in a case of AML with p53 mutation. CONCLUSION: p16 gene may not play an important role in the pathogenesis of AML. The high p16 expression in the patient with p53 mutation might reflect a kind of inbalance of the feedback cycle.