复圣散对神经细胞缺血再灌注样损伤的影响

目的:观察复圣散对神经细胞的直接保护作用,进一步在细胞水平阐明复圣散治疗血管性痴呆的机理。方法:选用乳鼠原代神经细胞,采用"缺氧—复氧"结合"缺糖—高糖"的方法,对其进行攻击,模拟整体实验中神经细胞的缺血再灌注损伤。观察含复圣散的血清对此损伤的治疗作用。结果:"缺氧、缺糖"5h及"缺氧、缺糖"5h结合"复氧、高糖"5h对神经细胞均可造成明显损伤。导致细胞内丙二醛含量明显增加、超氧化物歧化酶活性明显下降,细胞膜流动性明显下降,细胞内钙含量明显升高。而含复圣散血清可以显著减轻上述损伤。结论:复圣散对"缺血再灌注"的神经细胞有直接保护作用,这可能是其对血管性痴呆治疗作用的机制之一。

Effect of Fu-Sheng powder on ischemia-reperfusion-like injury in neural cells

AIM: To further elucidate the mechanism underlying the protective effect of Chinese herb Fu Sheng powder on vascular dementia. METHODS: Primary passage of neural cells of new born rats were subjected to the ischemia-reperfusion-like injury of hypoxia plus glucose deprivation followed by reoxygenation plus glucose, and the effect of "Fu-Sheng powder" on neural cells was examined. RESULTS: Both 5 hours of "ischemia" and 5 hours of "ischemia" plus 5 hours of "reperfusion" led to severe injury to neural cells, the formation of MDA and intracellular calcium concentration increased significantly, however, the activities of SOD and fluidity of neurons decreased significantly. It was also observed that Fu-Sheng powder could significantly alleviate this injury. CONCLUSION: Fu-Sheng powder had direct protective effect on neurons subjected to iscehmia-reperfusion-likeinjury, which might be one of the mechanisms underlying its therapeutic effect on vascular dementia.