HPLC法同时测定黄芪中4种黄酮类成分的含量

目的:建立以高效液相色谱法同时测定11个产地黄芪中毛蕊异黄酮葡萄糖苷、芒柄花苷、毛蕊异黄酮和芒柄花素4种黄酮类成分含量的方法,从有效成分含量探讨药材道地性内在因素。方法:色谱柱为Zorbax Eclipse XDB-C1(8250mm×4.6mm,5μm),流动相为乙腈-0.2%甲酸水溶液(梯度洗脱),检测波长为280nm。结果:毛蕊异黄酮葡萄糖苷、芒柄花苷、毛蕊异黄酮和芒柄花素的检测浓度分别在0.0051～0.510、0.0050～0.300、0.0049～0.294、0.0046～0.276mg·mL-1范围内与各自峰面积积分值呈良好的线性关系(r均为0.9999)。内蒙古、山西等4个产地的黄芪中毛蕊异黄酮苷和芒柄花苷的含量较高;河北安国、赤城和甘肃定西产黄芪中毛蕊异黄酮和芒柄花素含量较高。结论:本方法简便、快速、准确,试验结果与黄芪本草考证、道地沿革的文献基本相符。

Simultaneous Determination of 4 Kinds of Flavonoids in Radix Astragali by HPLC

OBJECTIVE：To develop an HPLC method for simultaneous determination of calycosin-7-O-β-D-glycoside,ono-nin,calycosin and formononetin in Radix Astragali from eleven different habitats and to explore the internal factors of geoherbalism based on aspect of contents of active constituents. METHODS：The sample was separated on Zorbax Eclipse XDB-C18（250 mm×4.6 mm,5 μm）column. The mobile phase consisted of acetonitrile-0.2% formic acid solution （gradient elution）. The UV detection wavelength was set at 280 nm. RESULTS：The linear ranges of calycosin-7-O-β-D-glycoside,ononin,calycosin and formononetin were 0.005 1～0.510 mg·mL-1,0.005 0～0.300 mg·mL-1,0.004 9～0.294 mg·mL-1,0.004 6～0.276 mg·mL-1,respectively（r=0.999 9）.Calycosin-7-O-β-D-glycoside and ononin took up a big proportion in Radix Astragali from Inner Mongolia and Shanxi; calycosin and formononetin took up a big proportion in Radix Astragali from Anguo,Chicheng of Hebei province and Dingxi of Gansu province. CONCLUSION：The method is simple,rapid and accurate. Results of study are in line with textual research on Radix Astragali.