T cell lines selected with synthetic peptides are highly encephalitogenic in SJL/J mice |
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Authors: | D N Bourdette A A Vandenbark G Hashim R H Whitham H Offner |
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Affiliation: | Neuroimmunology Research, VA Medical Center, Portland, Oregon 97201. |
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Abstract: | T cell lines were selected from basic protein (BP)-immunized SJL/J mice using synthetic peptides encompassing the major SJL/J encephalitogenic determinant. Synthetic peptide-derived T cell lines proliferated in response to BP, the 89-169 peptidase fragment of BP and the synthetic peptides, pM87-99, pM90-99 and pM91-99. These lines transferred a demyelinating and chronic relapsing form of experimental autoimmune encephalomyelitis (EAE) into naive mice, and EAE induced by synthetic peptide-derived lines was more severe than that induced by whole BP-derived lines. This study demonstrates that T cell lines selected with synthetic peptides are encephalitogenic in SJL/J mice and offers an improved means for selecting SJL/J encephalitogenic T cell lines. |
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