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| 幽门螺杆菌分离培养技术的改进 | |
| 引用本文： | 黄庆华,佟素香,廖方,余振东,彭颖,许迪,叶嗣颖,谢小平. 幽门螺杆菌分离培养技术的改进[J]. 华中科技大学学报(医学版), 2001, 30(2): 145-147 |
| 作者姓名： | 黄庆华 佟素香 廖方 余振东 彭颖 许迪 叶嗣颖 谢小平 |
| 作者单位： | 1. 华中科技大学同济医学院基础医学院微生物学教研室, 2. 华中科技大学同济医学院附属协和医院检验科, 3. 华中科技大学同济医学院附属协和医院胃肠病研究室, |
| 基金项目： | 卫生部科研基金资助项目(No.98-1-123) |
| 摘 要： | 探讨自制的改良布氏活性炭巧克力培养基,置厌氧罐蜡烛培养环境分离临床粘膜标本幽门螺杆菌(Hp)的检出率.结果表明66例粘膜标本中分离出Hp54株(81.8%),44株Hp滤液有24株(54.5%)具有细胞毒活性,其中26例消化性溃疡患者有18例(69.2%),18例慢性胃炎患者有6例(33.3%)为Hp(Toxin+)感染.结果提示通过对Hp生物学性状和毒素的检测结果证实,该法可用于临床粘膜标本Hp的常规分离培养. |
| 关 键 词： | 培养基 厌氧罐蜡烛培养 幽门螺杆菌 |
| 修稿时间： | 1999-01-22 |
Improvement of Isolationand Culture Technique for Helicobacter Pylori |
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| Huang Qinghua ,Tong Suxiang ,Liao Fang et al. Improvement of Isolationand Culture Technique for Helicobacter Pylori[J]. Journal of Huazhong University of Science and Technology(Health Sciences), 2001, 30(2): 145-147 | |
| Authors: | Huang Qinghua Tong Suxiang Liao Fang et al |
| Affiliation: | Huang Qinghua 1,Tong Suxiang 2,Liao Fang 1 et al 1 Department of Microbiology,School of Basic Medical Sciences,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030 2 Department of Laboratory,Xiehe Hosp |
| Abstract: | The home made modified Brucella activated charcoal chocolate agar was put into an anaerobic can candle culture condition to detect the rate of Helicobacter pylori(Hp) of isolating culture from biopsied mucosa samples The results showed that 54 strains Hp were isolated from 66 mucasa samples (81 8 %) A total of 24 (54 5 %) out of 44 Hp filtrates produced a cytotoxic activity against human endothelium cell in vitro Hp (Toxin ) infection existed in 18 cases (69 2 %) out of 26 patients with digestive ulcer and 6 (33 3 %) out of 18 patients with chronic gastritis, respectively The study showed that it is confirmed through the measurement of biologic characters and cytotoxic activity of the isolated Hp that improvement of isolation and culture technique was an effective method and could be used as routinely isolating culture of Hp for biopsy mucosa samples |
| Keywords: | medium anaerobic can candle culture Helicobacter pylori |
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