TLR4基因多态性与慢性胃炎幽门螺杆菌感染无相关性

目的:研究我国湖北汉族人群TLR4基因 Asp299Gly多态性与慢性浅表性胃炎及幽门螺杆(H pylori)感染的关系．方法:采用病例-对照研究和多聚酶链反应-限制性片段长度多态性(PCR-RFLP)方法,检测 115例慢性浅表性胃炎患者115例和正常对照者2644例的TLR4等位基因Asp299Gly基因型分布．结果:慢性浅表性胃炎患者的H pylori阳性率 89．6％,显著高于正常对照组61．7％(P<0．000 1, OR=5．319．95％CI:2．784-10．162)．在H pylori 感染相关性的慢性胃炎组和正常对照组中 TLR4基因Asp299Gly基因型所有个体均为 AA纯合子,未发现的突变型,其基因型、等位基因以及携带者频率总体分布无显著性差异．结论:TLR4基因Asp299Gly基因多态性与H pylori相性慢性胃炎无明显相关性．

No association between Toil-like receptor-4 gene polymorphism and Helicobacter pylori infection in chronic gastritis

Aims: To study the distribution of Toll-like receptor-4 (TLR4) gene Asp299Gly poly-morp-hism and to define association between TLR4 genotype and Helicobacter Pylori asso-ciated chronic gastritis in Han Chinese of Hubei province. Methods: One hundred and fifteen patients with chronic superficial gastritis and 264 healthy controls were genotyped by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method for TLR4 gene Asp299Gly polymorphism. Meanwhile, H pylori infection was also detected in all the individuals. Results: The rate of H pylori infection was 89.6% in patients with chronic superficial gastritis, and 61.7% in the healthy controls, and there was significant difference between them (P < 0.000 1, OR = 5.319, 95% CI: 2.784-10.162). All the individuals had the same TLR4 Asp-299Gly genotypes (AA). The distributions of the genotypes and allele frequencies of TLR4 Asp299Gly were not significantly different between gastritis patients and healthy controls. Conclusion: There is no marked correlation between TLR4 Asp299Gly polymorphism and H pylori associated chronic gastritis in Han Chinese of Hubei province.