| 应用组织芯片技术研究间变性大细胞淋巴瘤中ALK蛋白的表达和意义 |
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| 引用本文： | 季天海,李慧灵,蒋会勇,赵彤,余英豪. 应用组织芯片技术研究间变性大细胞淋巴瘤中ALK蛋白的表达和意义[J]. 福州总医院学报, 2008, 0(Z1) |
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| 作者姓名： | 季天海 李慧灵 蒋会勇 赵彤 余英豪 |
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| 作者单位： | 福州总医院病理科;南方医科大学附属南方医院病理科; |
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| 基金项目： | 广东省社会发展攻关基金资助项目(B30301); 广州市科技计划基金资助项目(2002Z34061) |
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| 摘 要： | 目的:比较荧光原位杂交(fluorescence insitu hybridization,FISH)和免疫组织化学在检测间变性大细胞淋巴瘤(anaplasticlargecelllymphoma,ALCL)中间变性淋巴瘤激酶(anaplasticlymphomakinase,ALK)基因转位及其融合蛋白中的作用。方法:采用双色FISH和免疫组织化学检测30例石蜡包埋ALCL病例中ALK基因转位及其融合蛋白。结果:FISH和免疫组织化学分别在60.7%(17/28)、71.4%(20/28)系统性ALCL中检测到ALK基因转位或融合蛋白,在2例皮肤原发ALCL中均未检测到基因转位或融合蛋白。结论:在检测ALCL中有无ALK基因转位时,ALK蛋白免疫组化由于其简单、快捷、价廉成为一般情况下的首选方法;在具备FISH条件时,由于其准确性也可以将检测ALK基因转位作为首选。
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| 关 键 词： | 间变性大细胞淋巴瘤 间变性淋巴瘤激酶 荧光原位杂交 免疫组织化学 |
Investigate the expression of ALK protein in anaplastic large cell lymphoma by tissue microarray technique |
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| Ji tian-hai Li hui-ling Jiang hui-yong Zhao tong Yu ying-bao.. Investigate the expression of ALK protein in anaplastic large cell lymphoma by tissue microarray technique[J]. Journal of Fuzhou General Hospital, 2008, 0(Z1) |
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| Authors: | Ji tian-hai Li hui-ling Jiang hui-yong Zhao tong Yu ying-bao. |
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| Affiliation: | Ji tian-hai Li hui-ling Jiang hui-yong Zhao tong Yu ying-bao.(Department of Pathology,Fuzhou general Hospital of PLA.Fuzhou 350025,China) |
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| Abstract: | Objective:To compare the value of fluorescence insitu hybridization(FISH)with that of immunohistoche- mistry in detecting ALK gene translocations and ALK fusion protein in anaplastic large cell lymphoma(ALCL).Method:Dual- color FISH and ALK-1 immunohistochemistry were used to detect ALK gene translocation and ALK fusionproteln in 30 ALCL cases.Results:ALK gene translocations and ALK fusion protein expression were detected in 17 or 20 of the 28 systemic ALCL; it was not detected in 2 primary cutaneous ALCL.C... |
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| Keywords: | anaplastic large cell lymphoma anaplasticlymphomakinase fluorescence insitu hybridization immunohistochemical |
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