甲状腺滤泡性肿瘤DNA含量与AgNOR计数研究

目的：探讨DNA含量与AgNOR对甲状腺良恶性滤泡性肿瘤的鉴别诊断价值。方法：应用流式细胞术和胶体银染技术对9例正常甲状腺组织和36例滤泡性肿瘤（22例腺瘤和14例腺癌）进行DNA含量分析和AgNOR计数。结果：14例滤泡性癌中，11例为DNA异倍体，而22例滤泡性腺癌仅1例为DNA异倍体，且伴有不典型增生。AgNOR由正常甲状腺组织、滤泡性腺癌至滤泡性癌逐渐增加，相互之间有显著性差异（P＜0．05）；但各组间AgNOR计数有重叠，且DNA含量与AgNOR无明显相关性。结论：尽管AgNOR染色方法简便。经济，但在区别良恶性滤泡性肿瘤方面，DNA含量异常更具意义。

Study on DNA Content and AgNOR Count in the Follicular Neoplasm of the Thyroid Gland

To assess the diagnostic valoc of the DNA content and AgNOR in the distinchon of thethyorid follicular carcinoma from adenoma .Methods:DNA content and AgNOR coun in 9 normal thyroid tissue and 36 thyroid neoplasms (22 follicular adenomas and 14 follicular carcinomas ) were analysed byflow cytometry and silver colloid techmque ,Results : 11 of 14 carcinomas were DNA aneuploidy ,while inthe 22 cases of adenomas only 1 was DM aneuploidy with dysplasia. AgNOR increased obviously alongwith developent of the lesion . A significant difference was found among various groups (P < 0.05),butthere was also a considerable overlap between carcinoma and adenoma There was no corelationship between the DNA conten and AgNOR count . Conclusion: Althougb silver colloid technoque was simple , convenien and economical , the DNA aneuploidy was a possible aid in the distinction of the thyroid follicularcarcinoma from adenoma .