VEGF、IL-6的水平与糖尿病视网膜病变关系的研究

目的:检测2型糖尿病患者眼房水中血管内皮生长因子(Vascular endothelial growth factor,VEGF)和白细胞介素-6(Interleukin-6,IL-6)的含量,并探讨其临床意义.方法:在白内障手术过程中获取66例2型糖尿病患者的房水,采用双抗体夹心酶联免疫吸附(ELISA)法测定VEGF和IL-6的含量.根据手术后散瞳眼底检查和眼底荧光素血管造影检查确定糖尿病视网膜病变的分期.实验组分为:无糖尿病视网膜病变组(NDR)21例、单纯型糖尿病性视网膜病变组(BDR)26例、增生型糖尿病性视网膜病变组(PDR)19例,正常对照组为健康的老年性白内障患者20例.结果:NDR组、BDR组、PDR组的房水VEGF含量分别为(240.30±26.15)pg/ml、(292.27±58.91)pg/ml、(477.41±91.01)pg/ml,IL-6含量分别为(160.83±33.41)pg/ml、(238.60±62.23)pg/ml、(389.13±90.35)pg/ml,对照组房水VEGF含量为(140.58±26.27)pg/ml、IL-6含量为(82.72±21.53)pg/ml,对照组与实验组比较差异均有统计学意义(F=113.67,P＜0.01;F=106.53,P＜0.01).实验组房水中的VEGF与IL-6含量有相关性(r=0.995,P＜0.01);糖尿病患者的病程与房水中VEGF(r=0.792,0.826,0.841均P＜0.01)、IL-6(r=0.829,0.817,0.896均P＜0.01)含量有相关性.结论:VEGF、IL-6在糖尿病视网膜病变的形成过程中有重要作用,且两者之间有相关性.

Level of vascular endothelial growth factor and interleukin-6 in aqueous humor in diabetic retinopathy patients

PURPOSE:To explore the role of vascular endothelial growth factor and interleukin-6 in the aqueous humor in the pathogenesis of diabetic retinopathy(DR). METHODS:Aqueous humor samples were collected in type 2 diabetic patients during cataract surgery. The classification of DR was assigned based on the fundus examination and fluorescein angiography after cataract surgery. The experiment group included non-diabetic retinopathy (NDR,21 cases),background diabetic retinopathy (BDR,26 cases) and Proliferative diabetic retinopathy (PDR,19 cases). The control group was the healthy cataract patients (20 cases). The levels of VEGF and IL-6 in the aqueous humor was assessed using the ELISA technique. RESULTS:The level of VEGF in NDR,BDR and PDR cases were(240.30+/-26.15)pg/ml, (292.27+/-58.91)pg/ml and (477.41+/-91.01)pg/m1, respectively. The level of IL-6 in NDR, BDR and PDR cases were (160.83+/-33.41)pg/ml, (238.60+/-62.23)pg/ml and (389.13+/-90.35)pg/ml,respectively. The levels of VEGF and IL-6 in the aqueous humour from the control group were (140.58+/-26.27) pg/ml,(82.72+/-21.53)pg/ml.The level of VEGF and IL-6 were significantly different in experiment and control groups(F=113.67,p<0.01; F=106.53, p<0.01). In the aqueous humor of diabetic patients increased significantly(p<0.O1). The level of VEGF and IL-6 was significantly correlated with each other in experiment group (r=0.995,p<0.O1). The levels of VEGF(r=0.792,0.826,0.841,p<0.01)and IL-6(r=0.829, 0.817.0.896,p<0.01)were also significantly correlated with the duration of DR. CONCLUSIONS: VEGF and IL-6 play important roles in the development of DR. The levels of these two factors are correlated with each other in DR patients.