苦荞壳提取物对大鼠血脂的调节作用

目的:观察苦荞壳提取物对高脂血症大鼠血脂的调节作用,探讨其机制. 方法:将雄性Wistar大鼠50只按体质量随机分为普通饲料组、高脂模型组、苦荞壳提取物高剂量组(0.825 g/kg)、低剂量组(0.165 g/kg)和力平之对照组(0.067 g/kg). 于实验后第12周测定大鼠血清Ch, Tg, HDL-C, LDL-C, MDA含量, SOD, GPX活力和肝指数,并观察大鼠肝组织病理变化. 结果:高脂模型组大鼠血清Ch, Tg, LDL-C, MDA含量和肝指数显著高于普通饲料组(P<0.05); HDL-C, SOD显著低于普通饲料组(P<0.05); GPX低于普通饲料组,体质量高于普通饲料组,但无统计学差异(P>0.05). 力平之组大鼠血清Ch, Tg, LDL-C, MDA含量显著低于高脂模型组(P<0.05);GPX显著高于高脂模型组(P<0.05); HDL-C, SOD, 肝指数和体质量与高脂模型组之间无统计学差异(P>0.05). 苦荞壳提取物各组大鼠血清Ch, Tg, LDL-C, MDA和肝指数显著低于高脂模型组(P<0.05); SOD, GPX显著高于高脂模型组(P<0.05); HDL-C高于高脂模型组,体质量低于高脂模型组,但无统计学差异(P>0.05). 结论:苦荞壳提取物可降低实验性高血脂血症大鼠的血脂和肝指数,提高血液的抗氧化能力,减轻高脂饮食导致的氧化损伤.

Role of tartarian buckwheat shell extract in regulation of blood lipid in rats with hyperlipidemia

AIM: To investigate the anti-hyperlipidemia effect of tartarian buckwheat shell extract(TBSE) in rats and its mechanism.METHODS: Fifty male Wistar rats were randomly separated into 5 groups according to body weight.They were fed respectively with normal diet,high lipid diet,high lipid diet supplemented with different concentrations of TBSE(0.825,0.165 g/kg),high lipid diet supplemented with Lipingzhi capsules(LPZ,(0.067) g/kg).After 12 weeks,the concentrations of serum Ch,Tg, HDL-C,LDL-C,MDA,the activity of SOD and GPX,liver index were measured.RESULTS: Compared with those in normal diet group,the serum Ch,Tg,LDL-C,MDA and liver index in high lipid diet group increased significantly(P<0.05),the serum HDL-C and SOD decreased significantly(P<0.05).The serum GPX activity decreased and the body weight increased,but there was no significant statistical difference(P>0.05).In LPZ group,compared with those in high lipid diet group,the serum Ch,Tg,LDL-C and MDA decreased significantly(P<0.05),the serum GPX activity increased significantly(P<0.05),but the serum HDL-C,SOD,body weight and liver index had no significant statistical changes(P>0.05).Compared with those in high lipid diet group,the serum Ch,Tg,LDL-C,MDA and liver index in TBSE group decreased significantly(P<0.05),the(serum) GPX and SOD activity increased significantly(P<0.05).The serum HDL-C increased and the body weight decreased but there was no significant statistical difference(P>0.05).(CONCLUSION:) TBSE can reduce the blood lipid and liver(index) and enhance the blood antioxidant activity in rats with(hyperlipidemia,) which can protect the normal tissues against the injury induced by high lipid diet.