| HPLC法测定左金分散片中盐酸小檗碱的含量 |
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| 引用本文： | 曾彬,黄志芳,高剑坤,任劲松. HPLC法测定左金分散片中盐酸小檗碱的含量[J]. 亚太传统医药, 2012, 8(9): 13-14 |
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| 作者姓名： | 曾彬 黄志芳 高剑坤 任劲松 |
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| 作者单位： | 1. 四川中医药高等专科学校,四川绵阳,621000
2. 四川省中医药科学院,四川成都,610041 |
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| 摘 要： | 目的:建立测定左金分散片中盐酸小檗碱含量的高效液相色谱法。方法:色谱柱:PhenomenexLuna C18(150mm×4.6mm,5μm),流动相:乙腈-水-磷酸-十二烷基硫酸钠(540∶460∶0.9∶4.5g),流速:1mL/min,检测波长:344nm,柱温:35℃。结果:盐酸小檗碱的线性范围是0.063～0.315μg/mL,r=0.9999,平均回收率为99.37%,RSD为1.50%。结论:用该法测定左金分散片中盐酸小檗碱的含量,操作简单,重复性好,精密度高。
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| 关 键 词： | HPLC 盐酸小檗碱 左金分散片 含量测定 |
Determination of Berberine Hydrochioride in Zuojin Dispersible Tablets by HPLC |
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| Zeng Bin , Huang Zhifang , Gao Jiankun , Ren Jinsong. Determination of Berberine Hydrochioride in Zuojin Dispersible Tablets by HPLC[J]. Asia-Pacific Traditional Medicine, 2012, 8(9): 13-14 |
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| Authors: | Zeng Bin Huang Zhifang Gao Jiankun Ren Jinsong |
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| Affiliation: | 1. Sichuan college of Traditional Chinese Medicine, Mianyang, 621000, China ; 2. Sichuan Academy of Chinese Medicine Sciences, Chengdu 610041,China) |
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| Abstract: | Objective： To establish HPLC method for the determination of berberine hydrochloride in Zuojin dispersible tables. Methods：C18 colum（150mm× 4.6mm, 5μm）was used, with Acetonitrile-water-phosphoric acid-twelve sodium alkyl sulfate（540： 460： 0.9 ： 4.5g） as a mobile phase,the flow rate was lmL·min^-1 ,the detection wave length was at 344nm and the temperature of column was at 35 ℃. Results： The linear of berberine hydrochloride was in range of 0. 063 -0.315μg/mL, r =0. 9999. The average recovery was 99.37 % and RSD was 1.50 %, respectively. Conclusion ： The method is simple, rapid and accurate, and can be used for quality control of this preparation. |
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| Keywords: | HPLC Berberine Hydrochloride Zuojin Dispersible Tablets Quantitative Determination |
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