| 体外去SUMO化体系的建立及应用 |
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| 引用本文： | 王岳飞. 体外去SUMO化体系的建立及应用[J]. 河南大学学报(医学版), 2012, 0(2): 121-123,127 |
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| 作者姓名： | 王岳飞 |
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| 作者单位： | 上海交通大学医学院病理生理学教研室 |
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| 摘 要： | 目的构建体外去SUMO化系统用于药物筛选。方法大肠杆菌表达人Senp2催化活性结构域即Senp2C和SUMO1化的RanGAP1△C。结果 Ni 2+柱纯化获得有活力的酶Senp2C和底物蛋白质即RanGAP1△C-SUMO1。结论建立体外去SUMO化系统,可筛选影响去SUMO化活性的药物。
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| 关 键 词： | Senp2 SUMO1 去SUMO化 药物筛选 |
Establishment and application of an in vitro desumoylation system |
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| WANG Yue-fei. Establishment and application of an in vitro desumoylation system[J]. Journal of Henan University, 2012, 0(2): 121-123,127 |
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| Authors: | WANG Yue-fei |
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| Affiliation: | WANG Yue-fei(Department of Pathophysiology,School of Medicine,Shanghai Jiaotong University,Shanghai 200025,China) |
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| Abstract: | Objective To set up in vitro desumoylation system and use it to drug screening.Methods Senp2C was produce and SUMO-1-conjugated protein RanGAP1△C in Escherichia coli.Results Recombinant proteins were purified by Ni2+ columns.Conclusion In vitro desumoylation system is established successful and it can used in drug screening. |
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| Keywords: | Sentrin-specific protease 2 SUMO-1 Desumoylation Drug Screening |
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