| 反相高效液相色谱法测定榆黄酊剂中大黄素、大黄酚的含量 |
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| 引用本文： | 戴颖,江宝红,黄一平. 反相高效液相色谱法测定榆黄酊剂中大黄素、大黄酚的含量[J]. 亚太传统医药, 2012, 8(9): 25-27 |
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| 作者姓名： | 戴颖 江宝红 黄一平 |
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| 作者单位： | 1. 江苏省中医药研究院,江苏南京,210028
2. 南京中医药大学,江苏南京,210046 |
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| 基金项目： | 江苏省中医药局立项课题（L09068） |
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| 摘 要： | 目的:建立榆黄酊剂中大黄素、大黄酚的含量测定方法。方法:采用反相高效液相色谱法(RP-HPLC)法,色谱柱为Alltima C18(4.6mm×250mm,5μm);流动相为甲醇-0.1%磷酸溶液(78:22);流速为1.0mL·min-1;柱温35℃,进样量10μL。结果:大黄素在0.059～0.588μg/mL内峰面积积分值与进样量自然对数有良好的线性关系线,r=0.999 9;平均加样回收率为98.1%,RSD为1.25%,大黄酚在0.210～2.10μg/mL内峰面积积分值与进样量有良好的线性关系线,r=0.999 9;平均加样回收率为97.7%,RSD为1.27%。结论:该方法操作简单,准确,重复性好,可用于榆黄酊剂的质量控制。
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| 关 键 词： | 反高效液相 榆黄酊剂 大黄素 大黄酚 |
Determination of Emodin and Chrysophanol in Yuhuang Tincture by RP-HPLC |
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| Dai Wei , Jiang Baohong , Huang Yiping. Determination of Emodin and Chrysophanol in Yuhuang Tincture by RP-HPLC[J]. Asia-Pacific Traditional Medicine, 2012, 8(9): 25-27 |
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| Authors: | Dai Wei Jiang Baohong Huang Yiping |
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| Affiliation: | . Jiangsu Academy of Traditional Chinese Medicine, Jiangsu 210028,China; 2. Nanjing University of Chinese Medicine, Jiangsu 210046,China) |
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| Abstract: | Objective ： To determine astragaloside in Yu huang Tincture. Methods. The sanmple will determined on a Alltima C18 （4.6nm × 250ram, 5μm） by HPLC, the mobile phase consisted methanol-0.1% phosphoric acid solution（13：87）with flower rate at 1.0 mL·min^-1 and column temperature at 35℃, and injection voulm was 10μL. Results：The linear ranges of emodin was 0. 059- 0. 588μg/mL（r= 0. 999 9） ,with the average recovery were 98.1% （RSD1.25%）, The linear ranges of chrysophanol was 0. 210-2.10ffg/mL（r = 0. 999 9）,with the average recovery were 97.7% （RSD1.27%）. Conclusion： The method is simple, accurate, reproducible and it can be used for the quality control of Yu huang Tincture. |
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| Keywords: | RP-HPLC Yuhuang Tincture Emodin Chrysophanol |
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