| 肾毒血清肾炎大鼠肾组织中NF-κB活化及其意义 |
| |
| 引用本文： | 潘晓勤,张爱华,黄松明,费莉,郭梅,陈荣华. 肾毒血清肾炎大鼠肾组织中NF-κB活化及其意义[J]. 南京医科大学学报(自然科学版), 2003, 23(6): 533-535 |
| |
| 作者姓名： | 潘晓勤 张爱华 黄松明 费莉 郭梅 陈荣华 |
| |
| 作者单位： | 南京医科大学小儿肾脏病研究中心，南京医科大学小儿肾脏病研究中心，南京医科大学小儿肾脏病研究中心，南京医科大学小儿肾脏病研究中心，南京医科大学小儿肾脏病研究中心，南京医科大学小儿肾脏病研究中心 江苏 南京 210029，江苏 南京 210029，江苏 南京 210029，江苏 南京 210029，江苏 南京 210029，江苏 南京 210029 |
| |
| 基金项目： | 国家自然科学基金(30100081)，江苏省教育厅科研基金(99KJB320003) |
| |
| 摘 要： | 目的：探讨肾毒血清性肾炎大鼠肾组织核因子—κB(NF—κB)活化及其意义。方法：肾毒血清肾炎应用兔抗鼠肾小球基膜肾毒血清制备。应用凝胶电泳迁移率(EMSA)和Western blot检测。肾毒血清。肾炎大鼠。肾组织中NF-κB活化及IκBα和IκBβ的降解；采用核酸酶保护法检测肾组织中IL-8表达，并分析其与NF—κB活化的关系。结果：模型组肾组织中IL-8表达显著高于正常对照组；肾毒血清肾炎大鼠肾组织中NF—κB活化显著增强，p65由胞质转移至咆核．咆质内IκBα和IκBβ降解明显增加；NF-κB活化与IL-8表达呈显著正相关。结论：NF—κB／IκB信号通路介导小球肾炎肾组织中IL-8表达。
|
| 关 键 词： | 核因子-κB 肾小球肾炎 白细胞介素-8 |
| 文章编号： | 1007-4368(2003)06-0533-03 |
| 修稿时间： | 2003-03-28 |
Role of NF-κB/IκB Signal Pathway in the Expression of Interleukin-8 in Experimental Rat Glomerulonephritis |
| |
| PANXiao-qin,ZHANG Ai-hua,HUANG Song-ming,FEI Li,GUO Mei,CHEN Rong-hua. Role of NF-κB/IκB Signal Pathway in the Expression of Interleukin-8 in Experimental Rat Glomerulonephritis[J]. Acta Universitatis Medicinalis Nanjing, 2003, 23(6): 533-535 |
| |
| Authors: | PANXiao-qin ZHANG Ai-hua HUANG Song-ming FEI Li GUO Mei CHEN Rong-hua |
| |
| Abstract: | Objective: To investigate the role of NF-KB/lKB signal pathway in the experimental nephrotoxic sera nephritis of rats. Methods: Nephrotoxic sera nephritis (NTN) was prepared by injection of anti-GBM antibody in rats. Electrophoretic mobility shift assay (EMSA) and Western blot were used to detect the activity of NF-KB and degradation of 1KB. IL-8 expression was assessed by ribonu-clease protection assay. Results: NF-KB activation, nuclear translocation of p65 subunit, and degradation of iKBa and iKBpJ were significantly up-regulated in NTN rats compared to the control group, and so was the expression of IL-8. NF-KB activation was positively correlated with IL-8 expression. Conclusion: NF-KB /IxB signal pathway may play a pivotal role in pathogenesis of glomerulonephritis. |
| |
| Keywords: | nuclear factor-kappa B glomerulonephritis interleukin-8 |
| 本文献已被 CNKI 维普 等数据库收录！ |
