吗啡依赖对小鼠海马神经元核酸代谢的影响

目的：探讨吗啡依赖对小鼠海马神经元结构和功能损害的分子机制。方法：以剂量递增法皮下注射吗啡建立吗啡依赖小鼠模型，采用吖啶橙荧光探讨技术，显示吗啡领带组、纳洛酮催促戒断组和正常对照组小鼠海马神经元DNA和RNA的变化。结果：与对照组相比，吗啡依赖组和纳洛酮催促戒断组海马神经元DNA和RNA荧光反应强度均明显减弱，纳洛酮催促戒断组荧光反应强度更弱。结论：吗啡依赖和纳洛酮催促戒断损伤海马神经元核代谢，后者的损伤程度更为严重，这些变化可能是吗啡依赖造成脑功能损坏尤其是学习记忆功能下降的原因之一。

Effect of morphine dependence on nucleic metabolism in hippocampal neurons in mice

Objective To study the molecular mechanism about injurious effect of morphine dependence on the construction and function of hippocampal neurons. Method Mice were given (sc) increasing doses of morphine to form morphine dependence model ,and the DNA and RNA changes stained with acridine orange (AO) fluorescence probe technique were investigated in hippocampal neurons of morphine dependence group,naloxone precipitated withdrawal group in morphine dependence mice and control group. Results Compared with control group , the staining changes of DNA,RNA decreased obviously in hippocampal neurons of both morphine dependence group and naloxone precipitated withdrawal group in morphine dependence mice,especially the later. Conclusion Both morphine dependence and naloxone precipitated withdrawal in morphine dependence mice injured nucleic acid metabolism in hippocampal neurons ,especially the later.Those changes may be some reasons of decreased brain function ,especially in learning and memory deficits.