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The effect of bone marrow‐ and adipose tissue‐derived mesenchymal stem cell transplantation on myocardial remodelling in the rat model of ischaemic heart failure
Authors:Andrey A. Karpov  Yulia K. Uspenskaya  Sarkis M. Minasian  Maxim V. Puzanov  Renata I. Dmitrieva  Anna A. Bilibina  Sergey V. Anisimov  Michael M. Galagudza
Affiliation:1. Department of Pathophysiology, I.P. Pavlov Federal Medical University, , St‐Petersburg, Russia;2. Institute of Experimental Medicine, V.A. Almazov Federal Heart, Blood and Endocrinology Center, , St‐Petersburg, Russia;3. Institute of Molecular Biology and Genetics, V.A. Almazov Federal Heart, Blood and Endocrinology Center, , St‐Petersburg, Russia
Abstract:This study aimed to investigate the effect of bone marrow‐ and adipose tissue‐derived mesenchymal stem cell (BM‐MSC and AD‐MSC respectively) transplantation on left ventricular function and infarct area (IA) in the rat model of ischaemic heart failure. In anaesthetized Wistar rats, the left coronary artery (LCA) was occluded for 40 min with subsequent reperfusion for 7 days. Seven days following surgery, the animals with LCA occlusion/reperfusion were randomized into three groups: (i) Controls received intramyocardial injection of vehicle at three different locations within the peri‐infarct zone, (ii) BM‐MSC: cells were injected in the same way as in previous group (106), (iii) AD‐MSC: using the same protocol as used in the BM‐MSC group. In addition there was also a sham‐treated group that had no injection. Two weeks following MSC transplantation, the hearts were isolated and perfused according to the Langendorff method followed by 30‐min global ischaemia and 90‐min reperfusion. After this IA was determined histologically. During Langendorff perfusion initial and postischaemic LV functions were the same in all groups although LV pressure at the 10th minute of reperfusion was higher in the AD‐MSC group compared to controls. However, LV pressure during 30‐min global ischaemia was significantly higher in BM‐MSC as compared to controls and AD‐MSC. The sham treated animals showed the same results as those seen with BM‐MSC. Thus, BM‐MSC transplantation, in contrast to transplantation of AD‐MSC, resulted in better preservation of the LV ability to contract during ischaemia. Furthermore, IA was significantly smaller in BM‐MSC group as compared to the controls and the AD‐MSC groups. Thus this study has demonstrated that treatment with BM‐MSC both ameliorates LV function and reduces histological scar size.
Keywords:adipose tissue‐derived mesenchymal stem cells  bone marrow‐derived mesenchymal stem cells  chronic heart failure  myocardial infarction
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