IL-12对结构优化的HBV核心抗原DNA疫苗诱导免疫应答的影响

目的 探讨IL-12对一种结构优化的HBV核心抗原(HBcAg)DNA疫苗免疫效果的影响。方法 将小鼠IL-12基因插入结构优化的DKA疫苗pST-HBc，构成pST-HBc/IL12，将pST-HBc／IL12转染COS7细胞，ELISA检测培养上清中的HBcAg和小鼠IL-12。分别将pST-HBc／IL12和pST-HBc肌肉注射接种BALB／c小鼠，检测小鼠的体液和细胞免疫应答。结果 ELISA检测到培养上清液中的HBcAg和小鼠IL-12，pST-HBc／IL12诱导的抗-HBc总IgG阳转率和抗体水平不及pST-HBc，但其诱导的脾细胞增殖反应和细胞毒性T淋巴细胞反应均强于pST-HBc。结论 IL-12可进一步增强这种HBcAgDNA疫苗诱导的细胞免疫应答。

Impact to immune responses by interleukin 12 on optimized hepatitis B core antigen DNA vaccine in mice

Objective To observe the influence of immune responses stimulated with interleukin 12 (IL 12) on optimized hepatitis B core antigen (HBcAg) DNA vaccine. Methods Murine IL 12 cDNA was inserted into optimized HBcAg DNA vaccine pST HBc. The resultant recombinant expression plasmid pST HBc/IL12 was transfected into COS7 cells and its expressed product was detected by ELISA. BALB/c mice were immunized intramuscularly with pST HBc, pST HBc/IL12 or mock vector, respectively. The humoral and cellular immune responses of the mice were detected. Results pST HBc/IL12 could express secretory HBcAg and murine IL 12. In mice, pST HBc/IL12 induced weaker anti HBc IgG but stronger T lymphocyte proliferative response and cytotoxic T lymphocyte response than that of pST HBc did. Conclusion IL 12 enhanced cellular immune responses that were elicited by optimized HBcAg DNA vaccine. This result may be refered in the development of therapeutic vaccine against hepatitis B.