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| 溶纤法测定125I标记基因重组葡萄球菌激酶的活性 | |
| 引用本文： | 邝荔香,吕继红,陈敏珠,沈子龙. 溶纤法测定125I标记基因重组葡萄球菌激酶的活性[J]. 安徽医药, 2005, 9(5): 362-362 |
| 作者姓名： | 邝荔香 吕继红 陈敏珠 沈子龙 |
| 作者单位： | 1. 安徽医科大学临床药理研究所,安徽,合肥,230022 2. 西安中药集团,陕西,西安,710003 3. 中国药科大学海洋药物研究中心,江苏,南京,210008 |
| 摘 要： | 目的测定125I标记前后的基因重组葡萄球菌激酶(r-Sak)活性.方法采用抑菌圈原理,建立纤维蛋白平板溶纤法.结果在0.625～10 mg·L-1浓度范围线性良好(r=0.997),125I标记后r-Sak为标记前的81.1%.结论该方法简便准确,操作方便. |
| 关 键 词： | 基因重组葡萄球菌激酶 125I r-Sak 溶纤圈 溶纤圈直径 |
| 修稿时间： | 2004-09-14 |
The active determination of 125I r-Sak by method of solution fiber protein |
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| KUANG Li-xiang,LU Ji-hong,CHEN Ming-zhu,SHEN Zi-nong. The active determination of 125I r-Sak by method of solution fiber protein[J]. Anhui Medical and Pharmaceutical Journal, 2005, 9(5): 362-362 | |
| Authors: | KUANG Li-xiang LU Ji-hong CHEN Ming-zhu SHEN Zi-nong |
| Abstract: | Aim To determine 125I r-Sak activity.Methods Using theory of inhibit germ circle,building plate of solution fiber protein, the activity of r-Sak before and after 125I marked was tested.Results The method proved to be linear(r=0.997), r-Sak activity of after 125I marked was 81.1% of before. Conclusion The method is simple and accurate. |
| Keywords: | r-Sak 125I r-Sak solution fiber protein circle diameter of solution fiber protein circle |
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