金线莲与内生真菌共生培养体系的建立

目的 :建立金线莲与内生真菌共生培养体系 ,为研究内生真菌促进金线莲生长机理及金线莲栽培新方法奠定基础。方法 :在金线莲组培苗基础上 ,将金线莲苗与真菌共培养 ,定期观察记录金线莲苗与真菌的生长状态 ,根据菌 苗共生特点筛选适合金线莲与内生真菌共生的培养条件。结果 :建立了金线莲与内生真菌的共生培养体系。最适共生培养基为 (mg·L^-1) :NH₄NO₃825 ,KNO₃950 ,MgSO₄185 ,肌醇 10 0 ,其它有机成分为MS培养基有机成分量的 2 /3 ,蔗糖 15 g·L^-1,其它成分同MS培养基 ,琼脂 9g·L^-1,pH 5 .8。培养条件为培养温度 2 4～ 2 5℃ ,光照 15 0lx ,光照时间 11h。结论 :在此共生培养体系中金线莲苗与所筛选出的优良菌种可形成内生菌根 ,并可长期共生 ,且真菌有促进金线莲生长发育作用。

Establishment of Symbiotic System for Anoectochilus roxburghii (Wall.) Lindl. and Endophytic Fungi

OBJECTIVE: Setting up a symbiotic system for Anoectochilus roxburghii and endophytic fungi, so as to study the relationship between them and try to set up new cultural methods for A. roxburghii. METHOD: A. roxburghii and its endophytic fungi were cultured together on five kinds of media in flasks. The growth of the plantlets and fungi were observed. Symbiotic conditions were selected according to symbiotic characteristics. RESULTS: A symbiotic system for A. roxburghii and endophytic fungi was set up. The optimum composition of the system induded NH4NO3 825 mg.L-1, KNO3 950 mg.L-1, MgSO4 185 mg.L-1, and inositol 100 mg.L-1, other organic components being 2/3 times those of MS medium, sugrose 15 g.L-1, and other components being the same as those of MS medium, agar 9 g.L-1, pH 5.8. The culture was effected at 24-25 degrees C under cool white fluorescent light (150 lx) for a photoperiod of 11 hours. CONCLUSION: A. roxburghii and endophytic fungi can grow well together and form endomycorrhiza in the symbiotic system. Moreover, endophytic fungi help stimulate the growth and development of A. roxburghii.