桑寄生乙醇提取物抗柯萨奇病毒B3的实验研究

目的：为研制抗柯萨奇病毒B3(CVB3)新药，观察了桑寄生乙醇提取物不同部分抗CVB3药效。方法：以病毒唑为阳性对照，用空斑减数实验检测了抗CVB3药效。结果：在HEp-2细胞系统中，乙酸乙酯萃取部分(B)对CVB3直接杀灭、感染阻断、增殖抑制的ED50为2.32，0.24，1.91 μg．ml-1。正丁醇萃取部分(C)的相应数值分别为1.44，2.06，3.70 μg．ml-1。病毒唑对CVB3增殖抑制的ED50为7.55 μg．ml-1。结论：桑寄生B部分对CVB3直接杀灭、感染阻断、增殖抑制的治疗指数(TI)为22.6，219，27.5，C部分的相应TI为115，165，21.6。病毒唑对CVB3增殖抑制的TI为38，数值相近。桑寄生B，C部分抗CVB3作用值得开发。

Experimental Research on Inhibitory Effect of Alcohol Extracts from Loranthus yadoriki Sieb. on Coxsackie B3 Virus

OBJECTIVE: To investigate the antiviral effect of two components (B and C) in the alcohol extracts from Loranthus yadoriki Sieb for development of new antiviral drugs for coxsackie B3 virus(CVB3). METHOD: Using ribavirin as positive control, the plaque reduction assay was adopted for pharmadynamic detection. RESULTS: In the HEp-2 cell system, with respect to direct virucidal activity and the inhibition on CVB3 infection and multiplication, ED50s of component B were 2.32 micrograms.ml-1, 0.24 microgram.ml-1 and 1.91 micrograms.ml-1, respectively and those of component C, 1.44 micrograms.ml-1, 2.06 micrograms.ml-1 and 3.70 micrograms.ml-1 respectively. For the inhibition on multiplication of CVB3, ED50 of ribavirin was 7.55 micrograms.ml-1. CONCLUSION: Treatment indexes (TI) of the direct virucidal activity and inhibitory effects of component B on CVB3 infection and multiplication 22.6, 219 and 27.5 respectively; component C, 115, 165 and 21.6; inhibition of ribavirin on CVB3 is 38, which is comparable to those of components B and C. It is thus suggested that the anti-CVB3 action of components B and C in the alcohol extracts from Loranthus yadoriki Sieb deserves to be exploited.