骨膜成骨细胞与生物衍生骨材料联合培养的实验研究

目的探索山西省医用组织库生产的冻干人同种骨植入材料作为组织工程支架材料的可行性。方法取兔胫骨前骨膜的成骨细胞，传代培养后作为种子细胞与冻干人松质骨小粒在体外联合培养，通过倒置相差显微镜、光镜及扫描电镜观察，了解成骨细胞在人松质骨小粒支架材料中的生长情况。结果冻干人松质骨材料具有天然不规则网孔结构，体外复合培养3天，细胞贴敷伸展，个别部位有少量细胞增殖；培养1周，形成了由一层或几层细胞构成的细胞膜片，覆盖整个骨小梁网架表面；培养3周，所形成的细胞膜片已较厚，细胞表面有基质分泌。结论人同种骨为天然骨基质，具有良好的生物相容性及细胞吸附性，是细胞良好的载体材料，可作为骨组织工程中较好的支架材料。

Experimental study of periosteal osteoblasts coculture with human freeze-dried cancellous bone

Objective To investigate the feasibility of the human freeze-dried cancellous bone produced by Shanxi Provincial Tissue. Bank as bone scaffold material in bone tissue engineering. Methods Osteoblasts obtained from the frontal tibial periosteum of the rabbit were cultured and passed. The third passed cells were seeded on human cancellous bone. The cell-material complex was observed under phase microscope, light microscope and scanning electron microscope to evaluate the interaction between cells and human cancellous bone. Results Human freeze-dried cancellous bone had natural texture. Three days after coculture, the cells spread and multiplicated in some area. Seven days later, the cell sheets were formed through cell proliferation. Twenty-one days later, the cell sheets were much more thicker and extracellular matrix was secreted from the osteoblasts. Conclusion Human freeze-dried bone matrices are natural materials and have good histocompatibility and cell-absorbability. They are good cell carriers and good scaffold material for the bone tissue engineering.