白芍总甙对大鼠腹腔巨噬细胞产生肿瘤坏死因子的影响

采用Ｌ９２９细胞株细胞毒检测法，观察了白芍总甙（ＴＧＰ）对ＬＰＳ诱导大鼠腹腔巨噬细胞（ＭΦ）产主肿瘤坏死因子（ＴＮＦ）的影响。结果表明、亚适量ＬＰＳ（５ｍｇ·Ｌ－１）诱导大鼠腹腔ＭΦ培养上清在６ｈ对Ｌ９２９细胞杀伤百分率最大．ＴＧＰ（２．５ｍｇ·Ｌ－１）能促进ＬＰＳ诱导大鼠腹腔ＭＯ产生ＴＮＦ，但不改变其动力学过程。ＴＧＰ（０．５～２５０ｍｇ·Ｌ－１）对ＬＰＳ诱导ＭΦ产生ＴＮＦ量效曲线呈钟罩形．提示ＴＧＰ对ＭΦ产主ＴＮＦ具有浓度依赖性双向调节作用。不同浓度ＴＧＰ对Ｌ９２９细胞无直接杀伤作用，亦无ｒｈＴＮＦ－α拮抗作用。

Effect of total glucosides of paeony (TGP) on tumor necrosis factor produced by peritoneal macrophages in rats

The effect of TGP on tumor necrosis factor (TNF)produced by peritioneal macrophages (MΦs) in rats was studid by assay of cytotoxicity against L929cells. The results showed that the supernatants of MΦs incubated with suboptimal lipopolysaccharide (LPS.5 mg·L-1) at the first 6h had the maximal killing effect.TGP(2.5 mg·L-1)could enhance TNFproduced by LPS-induced MΦs. but not alter its kinetics. The concentration-effect curve of TGP-LPS for TNF release seems to be bell-shaped, suggesting that TGP has a dual immunomodulatory activity in a does dependent manner. TGP does not have direct killing effect on L929 cells, or antagonism on recombinant human TNF-α.