| 脐带间充质干细胞与许旺细胞共移植修复大鼠坐骨神经缺损 |
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| 作者姓名： | Jin-Yong Lee Hun-Jong Jung Mohammad S. Alrashdan Bohan Li Mi-Ae Sung Sang Bae Yoo Soung-Min Kim Myung-Jin Kim Jeong Won Jahng Jong-Ho Lee |
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| 作者单位： | Dept. of Oral and Maxillofacial Surgery, School of Dentistry, Seoul National University, Seoul, Korea,Dept of Occupation and Enviroment, Konkuk Postgraduate Medical School, Choong-Ju, Korea,Dept. of Oral and Maxillofacial Surgery, School of Dentistry, Seoul National University, Seoul, Korea,Dept. of Oral and Maxillofacial Surgery, School of Dentistry, Seoul National University, Seoul, Korea,Dept. of Craniofacial Structure and Functional Biology, Seoul National University, Seoul, Korea,Dental Research Institute, School of Dentistry, Seoul National University, Seoul, Korea,Dept. of Oral and Maxillofacial Surgery, School of Dentistry, Seoul National University, Seoul, Korea,Dept. of Oral and Maxillofacial Surgery, School of Dentistry, Seoul National University, Seoul, Korea,Dental Research Institute, School of Dentistry, Seoul National University, Seoul, Korea,Dept. of Oral and Maxillofacial Surgery, School of Dentistry, Seoul National University, Seoul, Korea |
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| 基金项目： | the Korea Healthcare Technol-ogy R&D Project, Ministry for Health, Welfare & Family Affairs, Republic of Korea, No. A101578 |
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| 摘 要： | Previous research has demonstrated that cotransplantation of umbilical cord mesenchymal stem cells (UCMSCs) and Schwann cells (SCs) can repair spinal nerve injury, but few studies have investigated their use in peripheral nerve regeneration. In the present study, we cotransplanted UCMSCs and SCs to repair 5-mm left sciatic nerve defects in rats, and compared the effects of UCMSCs + SCs transplantation with UCMSCs or SCs transplantation alone. After UCMSCs + SCs transplantation, nerve conduction velocity of the left sciatic nerve and gait were both improved. Retrograde tracing analysis demonstrated that the mean count of fluorogold-labeled neurons, as well as the mean axon count and axon density, were significantly greater in the left sciatic nerve after UCMSCs + SCs transplantation, compared with UCMSCs or SCs transplantation alone. Improvements in conduction velocity and increased sheath thickness in the left sciatic nerve were similar after UCMSCs transplantation and UCMSCs + SCs transplantation. These findings suggest that UCMSCs transplantation can promote the repair of sciatic nerve defects to some extent, but that combined UCMSCs + SCs transplantation has a significantly greater regenerative effect.
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| 关 键 词： | Schwann cells human umbilical cord mesenchymal stem cells peripheral nerve re-generation axotomy |
Peripheral nerve regeneration with cotransplantation of umbilical cord mesenchymal stem cells and Schwann cells in rat sciatic nerve defect |
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| Jin-Yong Lee,Hun-Jong Jung,Mohammad S. Alrashdan,Bohan Li,Mi-Ae Sung,Sang Bae Yoo,Soung-Min Kim,Myung-Jin Kim,Jeong Won Jahng,Jong-Ho Lee.Peripheral nerve regeneration with cotransplantation of umbilical cord mesenchymal stem cells and Schwann cells in rat sciatic nerve defect[J].Neural Regeneration Research,2011,6(7):485-493. |
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| Authors: | Jin-Yong Lee and |
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| Institution: | 1, 4 1Department of Oral and Maxillofacial Surgery, School of Dentistry, Seoul National University, Seoul, Korea 2Department of Occupation and Environment, Konkuk Postgraduate Medical School, Choong-Ju, Korea 3Department of Craniofacial Structure and Functional Biology, School of Dentistry, Seoul National University, Seoul, Korea 4Dental Research Institute, School of Dentistry, Seoul National University, Seoul, Korea |
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| Abstract: | Previous research has demonstrated that cotransplantation of umbilical cord mesenchymal stem cells (UCMSCs) and Schwann cells (SCs) can repair spinal nerve injury, but few studies have investigated their use in peripheral nerve regeneration. In the present study, we cotransplanted UCMSCs and SCs to repair 5-mm left sciatic nerve defects in rats, and compared the effects of UCMSCs + SCs transplantation with UCMSCs or SCs transplantation alone. After UCMSCs + SCs transplantation, nerve conduction velocity of the left sciatic nerve and gait were both improved. Retrograde tracing analysis demonstrated that the mean count of fluorogold-labeled neurons, as well as the mean axon count and axon density, were significantly greater in the left sciatic nerve after UCMSCs + SCs transplantation, compared with UCMSCs or SCs transplantation alone. Improvements in conduction velocity and increased sheath thickness in the left sciatic nerve were similar after UCMSCs transplantation and UCMSCs + SCs transplantation. These findings suggest that UCMSCs transplantation can promote the repair of sciatic nerve defects to some extent, but that combined UCMSCs + SCs transplantation has a significantly greater regenerative effect. |
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