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隐孔菌不同发酵培养菌丝与野生隐孔菌的糖类成分比较分析
引用本文:王政,陈丽艳.隐孔菌不同发酵培养菌丝与野生隐孔菌的糖类成分比较分析[J].海峡药学,1997,9(2):91-92.
作者姓名:王政  陈丽艳
作者单位:福建中医学院!厦门市中山医院(王政),福建中医学院(吴锦忠),福州市药品检验所(陈丽艳)
摘    要:本文采用3,5-二硝基水杨酸为显色剂进行比色,测定隐孔菌不同发酵培养的菌丝及野生隐孔菌的糖类成分,结果表明玉术粉或马铃薯发酵培养基培养的隐孔菌菌丝多糖含量(分别为21.48%和21.58%)均高于野生隐孔菌(17.45%)(P<0.05),但发酵培养的菌丝间多糖含量无明显差异(P>0.05);发酵培养过程中多糖合成是在菌丝中进行,而培养基以还原糖和低聚糖的形式向菌丝中运输;新鲜菌丝在烘干过程中菌丝中还原糖和低聚糖向多糖转化,但总含量不变。

关 键 词:隐孔菌  发酵培养  糖类  成分分析

Comparative Analysis of Sugar Content of Natural Cryptoporus volvatas(Peck)Schear with Its Hyphae Different Terment Cultivation
Wang Zheng, et al.Comparative Analysis of Sugar Content of Natural Cryptoporus volvatas(Peck)Schear with Its Hyphae Different Terment Cultivation[J].Strait Pharmaceutical Journal,1997,9(2):91-92.
Authors:Wang Zheng  
Institution:Wang Zheng; et al
Abstract:The Polysaccharides contents of cryptoporus volvatus (Peck) schear were determined by means of colorimetry with 3, 5-dinitro-salicylic acid as colorant. The results showed that in the fungi ferment hyphae with maize or potato as culture rnedium the polysaccharides content were 21. 48 % and 21. 58 % respectively,more than that of the natural one with containing 17. 45% (P<0. 05). In proceeding ferment cultivation, in the sugar for bio-synthesis polysaccharide transited from cultive medium as monosaccharide and oligosaccharide. There was the bio-synthesis in these hyphea. The monosaccharide and oligosaccharlde in fresh hyphea might be translated into synthesizing polysaccharide,but the total sugar content did not change.
Keywords:Cryptoporus volvatus(Peck)schear  Ferment cultivation  Sugar  Analysis
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