儿童哮喘患者血清Eotaxin、ECP的测定及其临床意义

目的 探讨嗜酸粒细胞趋化酞（Eotaxin）、嗜酸粒细胞阳离子蛋白（ECP）在儿童支气管哮喘发病机制中的作用。并评价其反映哮喘气道炎症的临床价值。方法 收集23例哮喘急性发作患者、22例哮喘缓解期患者及17名健康儿童的外周血标本。用ELISA法测定血清Eotaxin的水平。以Pharmacia CAP系统检测血清中ECP的含量。结果 哮喘急性发作患儿血清中Eotaxin、ECP水平明显高于缓解期患儿和健康对照组，Eotaxin、ECP水平的升高程度与病情严重程度相关，且两者相互之间呈正相关。结论 Eotaxin在哮喘的发病过程中对气道的嗜酸粒细胞炎症有影响作用。ECP可作为支气管哮喘气道炎症发生发展的重要指标之一。动态监测血清Eotaxin、ECP的变化。可以较好地反映气道嗜酸粒细胞炎症的反应过程。

The clinical significance of serum Eotaxin and ECP in children with bronchial asthma

Objective To explore the roles of Eotaxin and ECP in the pathogenesis of bronchial asthma and to evaluate their diagnostic value in asthmatic children. Methods Serum samples were obtained from 23 asthmatic children during acute attack, 22 children patients in remission and 17 normal children. ELISA, method was used to determine the concentrations of Eotaxin in serum. The concentrations of serum ECP was determined with Pharmacia CAP system. Results The serum Eotaxin and ECP levels in children with acute asthma was much higher than those in remission and normal children. The serum Eotaxin and ECP levels were associated with the severity of symptoms. And the level of Eotaxin was positively correlated with the level of ECP. Conclusion The serum levels of Eotaxin and ECP can be used to monitor the eosinophilic inflammatory process occurring in the asthmatic airways.