胶质母细胞瘤中MMPs和TIMPs的表达及其与瘤周水肿的相关性分析

目的:探讨胶质母细胞瘤中基质金属蛋白酶(MMPs)及其组织抑制物(TIMPs)的表达,并分析其与瘤周水肿的相关性。方法:用免疫组化方法检测胶质母细胞瘤中MMP-1、MMP-2、MMP-3、MMP-7、MMP-9和TIMP-1、TIMP-2的表达,并用原位分子杂交方法检测了MMP-9mRNA分布。结果:17例胶质母细胞瘤,MMP1全部阳性,MMP-9阳性10例,主要定位于大核细胞胞核和部分胞浆;MMP-2阳性14例,MMP-3阳性6例,均位于小核细胞和细胞外基质;MMP-7阳性11例,灶性分布于出血、坏死区的小核细胞;均无TIMP-1和TIMP-2表达。8例影像学表现有瘤周水肿组MMP-9蛋白和mRNA阳性率均高于无瘤周水肿组,P<0.05,但其表达量与瘤周水肿程度之间无明确的线性关系。结论:在胶质母细胞瘤中,存在不同的MMPs的表达和TIMPs的缺乏,其中MMP-9可能与瘤周水肿的产生相关。

Expression of the matrix metalloproteinases and the tissue inhibitors of metalloproteinases and their correlation analysis with the peritumor edema in glioblastoma

Objective:To investigate the expression of the Matrix Metalloproteinases(MMPs) and the Tissue Inhibitors of Metalloproteinases(TIMPs) in the glioblastoma, and analyze their correlation with the peritumor edema. Methods:Immunohistochmistry method was used to detect the expression of MMP-1, MMP-2, MMP-3, MMP-7, MMP-9 and TIMP-1, TIMP-2 in 17 cases of glioblastoma, and the in situ hybridization was used to detect the MMP-9 mRNA disribution. Results:MMP-1 positive in all the 17 cases and MMP-9 positive in 10 cases, both mainly sited in the nucleus and part of the plasma of the large nuclear cells; MMP-2 positive in 14 cases and MMP-3 positive in 6 cases, both sited in small nuclear cells and extracellular matrix; MMP-7 positive in 11 cases and locally sited in the small nuclear cells in the hemorrhagic and necrotic area; while there is no cases expresses TIMP-1 and TIMP-2. Both the ratios of MMP-9 protein and mRNA positive in the 8 cases with peritumor edema are higher than that in non-peritumor edema (P<0.05), but there is no linear correlation between the expression with the peritumor edema degrees. Conclusions:There are varied MMPs expression and TIMPs deficiency in glioblastoma, the MMP-9 is probably related to the appearance of peritumor edema.