小鼠卡氏肺孢子虫肺炎的实验研究

目的 :开展卡氏肺孢子虫的病原学、病理学、免疫学、分子生物学以及药物治疗的研究。方法 :给昆明小鼠腹股沟皮下注射醋酸可的松 1～ 2 mg/只 ,每周 2次 ,诱发小鼠卡氏肺孢子虫肺炎模型。结果 :用药 5周肺印片即可检出卡氏肺孢子虫包囊 ,8周后大部分小鼠可检出 ,总检出率为 68.75 %。姬氏染色肺印片可查见成熟包囊、未成熟包囊 ,形态典型 ,是显示肺印片中包囊的可靠方法。GMS染色是显示肺组织石蜡切片中包囊的可靠方法。结论 :小鼠是建立卡氏肺孢子虫动物模型的理想动物 ,并具有简便、经济等优点

Experimental study on Pneumocystis carinii pneumonia in mice

Objective: To develop the studies of the aetiology, pathology, immunology, molecular biology and medication of Pneumocystis carinii. Methods: Kunming Mice under were subcutaneously injected at dose of 1～2mg of cortisone acetate,twice per week,so that mice model of pneumocystis carinii pneumonia was induced. Results: Five weeks after administration of the drug, the cysts of pneumocystis carinii could be found in the pulmonary impression. such cysts could be detected in most mice 8 weeks later,and the total detectable rate was 68.75%. From the pulmonary impression by Giemsa's stain, mature and immature cysts with a typical form could be detected. Therefore, Giemsa's stain is a reliable method which showed the cysts in the pulmonary impression. Gomori-Grocott's methenamine silver (GMS) stain is a reliable method which showed the cysts in the paraffin section of lung tissue. Conclusion: mice might be the ideal animal for the production of animal model of Pneumocystis carinii and have both simple and economical advantages.