可溶性晚期糖基化终末产物受体及其基因多态性与2型糖尿病的易感性分析

目的 探讨可溶性晚期糖基化终末产物受体(sRAGE)水平及晚期糖基化终末产物受体(RAGE)基因单核苷酸位点多态性与2型糖尿病的关联。方法 选择184例2型糖尿病患者作为糖尿病组,190例健康体检者作为正常对照组。ELISA方法检测sRAGE水平,PCR-TaqMan-MGB探针检测rs2070600、rs1800624、rs1800625及rs184003基因分型。结果 糖尿病组sRAGE水平[M(P₂₅,P₇₅)]为1087.4(713.5,1213.4)pg/mL,正常对照组sRAGE为908.2(674.6,1107.1)pg/mL,两组差异有统计学意义(P<0.001)。糖尿病组与正常对照组间4个位点的最小等位基因频率分布均无统计学差异,且在基因型分析中也无阳性发现。线性相关分析表明,4个位点与sRAGE水平均无明显关联性。结论 sRAGE水平与2型糖尿病的发病相关,但RAGE位点多态性与糖尿病无明显关联。RAGE位点多态性与sRAGE水平无关联。

Association between receptor of advanced glycation end products gene polymorphisms,soluble receptor of advanced glycation end products and susceptibility of type 2 diabetes mellitus

Objective To estimate the level of soluble receptor of advanced glycation end product (sRAGE) in type 2 diabetes mellitus, as well as its association with single nucleotide polymorphism (SNP) of receptor of advanced glycation end product (RAGE). Methods A total of 184 patients with type 2 diabetes mellitus and 190 healthy controls were recruited. ELISA was applied to test sRAGE, and PCR-TaqMan-MGB probe was used for the genotyping of rs2070600, rs1800624, rs1800625 and rs184003. Results The level of sRAGE[M(P₂₅,P₇₅)] in type 2 diabetes mellitus patients was[1087.4(713.5,1213.4) pg/mL], which was significantly higher than that of the controls[908.2(674.6,1107.1 pg/mL)]. As to the allele analysis, no significant differences were observed in the minor allele frequency comparisons of the 4 SNPs, and no association was found between the 4 SNPs and sRAGE level in the linear regression. Conclusion The elevated level of sRAGE is associated with type 2 diabetes mellitus, but the polymorphism of RAGE is not. There is no significant association between polymorphism of SNPs in RAGE and sRAGE level.