| 中药密蒙花药材RP-HPLC指纹图谱研究 |
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| 引用本文： | 韩澎,崔亚君,郭洪祝,果德安. 中药密蒙花药材RP-HPLC指纹图谱研究[J]. 中国中药杂志, 2004, 29(10): 938-940 |
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| 作者姓名： | 韩澎 崔亚君 郭洪祝 果德安 |
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| 作者单位： | 1. 北京大学,药学院,生物技术室,北京,100083
2. 首都医科大学,中医药学院,北京,100013 |
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| 基金项目： | 国家科技部攻关课题 ( 99 92 9 0 1 2 9) |
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| 摘 要： | 目的 :运用现代RP HPLC色谱法建立中药密蒙花指纹图谱 ,为密蒙花药材的质控提供依据。方法 :采用InertsilODS-3 C18色谱柱 (46mm×250mm ,5μm) ,以乙腈 (含 01%TFA) 水 (含 01%TFA)系统梯度洗脱 ,流速为 1mL·min-1,在 254nm下检测 ,测定 10份不同产地密蒙花商品药材的指纹图谱。结果 :用梯度洗脱得到的色谱图中都可以达到较好分离。该分析方法具有很好的精密度、重现性和稳定性 ,共有峰的相对保留时间和相对峰面积的RSD均小于 5 % ,符合指纹图谱相关要求。结论 :可用密蒙花的HPLC指纹图谱有效控制其内在质量。
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| 关 键 词： | 密蒙花 指纹图谱 RPHPLC |
| 文章编号： | 1001-5302(2004)10-0938-03 |
| 收稿时间： | 2004-02-25 |
Studies on fingerprinting of Flos Buddleja by RP-HPLC |
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| HAN Peng ;CUI Ya-jun ;GUO Hong-zhu ;GUO De-an. Studies on fingerprinting of Flos Buddleja by RP-HPLC[J]. China Journal of Chinese Materia Medica, 2004, 29(10): 938-940 |
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| Authors: | HAN Peng CUI Ya-jun GUO Hong-zhu GUO De-an |
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| Affiliation: | School of Pharmacy, Peking University, Beijing 100083, China. |
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| Abstract: | OBJECTIVE: To establish fingerprinting of Flos Buddleja by using RP-HPLC for the quality control. METHOD: The HPLC condition was as follows: Inertsil ODS-3 C18 analytical column (4.6 mm x 250 mm, 5 microm), gredient eluation with MeCN (0.1% TFA)-H2O (0.1%TFA), flow rate 1.0 mL x min(-1), detection wavelength 254 nm. 10 commercial samples were analyzed to establish a fingerprinting. RESULT: Among the obtained fingerprinting, most of the detected peaks were separated effectively. The accuracy, repeatability and stability of this method were satisfied. The RSDs of relative retention time and area of aimed peaks which existed in all samples wereless than 5%. Theresults were in accordance with the request of fingerprinting. CONCLUSION: The established fingerprinting can be used for the quality control of Flos Buddleja. |
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| Keywords: | Flos Buddleja fingerprinting RP-HPLC |
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