督脉电针对脊髓损伤大鼠神经干细胞的作用

目的　观察督脉电针对脊髓损伤后大鼠脊髓巢蛋白 (Nestin)表达和后肢功能的影响。方法　于术后 1、7、14、2 1和 2 8d分别对大鼠进行Tarlov评分和斜板试验检查后肢功能后处死动物 ,应用免疫组织化学技术检测神经上皮干细胞蛋白(Nestin)的表达 ,采用计算机图像分析仪进行定量分析 ,观察督脉电针对脊髓损伤后神经上皮干细胞蛋白的影响。结果　脊髓损伤后第 1d ,在督脉电针组和单纯脊髓损伤组的损伤脊髓灰质中都可见到Nestin的表达。单纯脊髓损伤组在术后第 7、14、2 1和 2 8d ,Nestin阳性细胞数分别为 2 1 4 8± 7 83、11 78± 4 38和 9 18± 3 2 6 ,而督脉电针组分别为 30 6 9± 6 16、39 2 4± 6 83、2 6 4 9± 5 87和 2 2 30± 6 6 1,两组在 4个时间段上均有显著性差异 (P <0 0 5 )。增加的Nestin阳性细胞数与神经功能的改善平行。结论　提示督脉电针可减轻神经损伤症状和促进脊髓损伤后神经干细胞的增殖。

Effect of Du Channel electrotherapy on neural stem cell following injuried spinal cord

Objective To investigate the effect of Du Channel electrotherapy on neural stem cell following injured spinal cord as well as functional recovery in the rats. Methods After operation open field and inclined plane texts were performed to text the function of the rear limbs. The animals were sacrificed at 1,7,14,21 and 28 day after surgery. Detect expression of the neuroepithelial stem cell protein,Nestin by immunohistochemical method,the effects of Du Channel electrotherapy on neural stem cell following injured spinal cord were observed. Results The Nestin positive expression cells were detected in the gray matter one day after spinal cord injury(SCI). At 7,14,21 and 28 day after surgery,The number of the Nestin positive expression cells was 21 48±7 83,11 78±4 38,9 18±3 26 in group A; 30 69±6 16,39 24±6 83,26 49±5 87,22 30±6 61 in group B(P<0 05). The increase in the nestin positive cells was paralleled by a significant improvement in neurological function evaluated by open field and inclined plane text. Conclusion Du Channel electrotherapy can alleviate the neurological symptons and promote the proliferation role of neural stem cell following spinal cord injury of rats.