AMG-1对小鼠和大鼠缺血脑能量代谢及神经细胞损伤的影响

本文观察AMG-1对小鼠断头全脑缺血时能量代谢及大鼠大脑中动脉阻断(MCAO)后行为和病理改变影响。小鼠sc AMG-1 1～10mg/kg 30min后,能有效减少脑缺血后乳酸(LA)堆积,ATP和磷酸肌酸(PCr)的耗竭。sc AMG-1 5mg/kg和尼英地平0.5 mg/kg,可减轻大鼠MCAO后的神经症状和神经细胞缺血性损害。AMG-1的这一改善脑缺血作用与尼莫地平近似。

EFFECTS OF AMG-1 ON ENERGY METABOLISM AND NEURONAL DAMAGE OF ISCHEMIC BRAIN IN MICE AND RATS

The effect of AMG- 1 6( 5 - hydroxy- 2- methylpyridylamino ) - 9 ribofranosylpurine] on mouse brain energy metabolism in complete brain ischemia induced by decapitation and on neurological deficit and histopathological changes after occlusion of the middle cerebral artery (MCAO) in rats were studied. The results indicate that AMG-1 has an effect of improving the status of energy metabolism in complete brain ischemia in mice. Lactate concentration was greatly reduced and the ATP and phosphocreatine levels were significantly elevated. Treatment with AMG-1 or nimodipine was performed before and after MCAO. The score of neurological deficit was significantly decreased as compared with the vehicle treated group. The extent of ischemic neuronal injury was determined by histopathological examination. AMG- 1 and nimodipine seemed to attenuate the MCAO - induced neuronal damage. In as much as AMG- 1 can improve the status of brain energy metabolism, neurological deficit and neuronal damage after ischemic insult, AMG- 1 may have a beneficial effect for the treatment of cerebral ischemic damage.