大豆异黄酮对去卵巢大鼠脂类代谢和抗氧化作用的影响

目的:探讨大豆异黄酮(SI)对去卵巢大鼠血清脂类代谢和脂蛋白(a)及抗氧化指标的影响。方法:将63只雌性SD大鼠摘除双侧卵巢,术后恢复1周,随机分为高脂对照组(HF组)、雌激素高脂饲料组(EG组)、低剂量SI高脂饲料组(L-SI组)、中剂量SI高脂饲料组(M-SI组)、高剂量SI高脂饲料组(H-SI组)、假手术组(Sham组)和正常对照组(NG组)共7组,灌胃给药3个月,每周称重一次,分别在大鼠去卵巢时、给药4、8、12 w抽取尾静脉血(每次采尾血前空腹12 h),分别测定总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)、脂蛋白aLp(a)]、抗氧化指标及骨密度等,实验结束后取内脏及骨骼标本。结果:SI各干预组与干预前比较TC、HDL-C、LDL-C显著增高(P均<0.01),H-SI组Lp(a)低于干预前(P<0.05)。SI干预后不同组间TG、LDL-C、Lp(a)改变显著(P均<0.01),LDL-C项各干预组明显低于HF组,但高于Sham组。干预后各组超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-PX)活性均有升高(P均<0.01)。结论:适量SI对去卵巢大鼠的高脂血症存在拮抗作用并能改善体内的脂质过氧化,尤其在应激阶段效果更佳。

Dynamic state influence of Soybean isoflavone on lipid metabolism and antioxidation in ovariectomized female rats

Objective:To study the influence of soybean isoflavone in lipid metabolism and antioxidation for ovariectomized rats.Methods:The 63 female SD rats after ovariectomized 1 week were randomly divided in to hypsi-lipoids(HF),estrogen,low,middle,high dose,sham operation,normal control groups according to the cholesterol level and intragastric administration with different dose of soybean isoflavone for 3 months.Weight rats ad unam vicem per week and measured about cholesterol(TC),triglyceride(TG),high,low density lipoprotein(HDL,LDL-C),lipoprotein a(Lp(a)),ossature,antioxidation each time after 4,8 and 12 weeks of the female SD rats were ovariectomized.Results:The level of serum TC,HDL-C,LDL-C were higher(P<0.01) and the Lp(a) was lower(P<0.01,in H-SI group) after the rates intervented by soybean isoflavone,the level of serum LDL-C were lower in the HF groups than that in normal control groups,but it was higher than Sham groups.The activity of erythrocuprein(SOD)and glutathion peroxidase(GSH-PX)had highed in intervention groups.Conclusion:Quantity sufficient soybean isoflavone could be effect antagonistic action hyperlipoidemia and improve lipid peroxidation state of ovariectomized rats,except in the stress phase.