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金标免疫层析法与间接血凝法在鼠疫诊断中的比较
引用本文:祁美英,王丽,于守鸿,谢辉,王梅,赵小龙,杨汉青. 金标免疫层析法与间接血凝法在鼠疫诊断中的比较[J]. 地方病通报, 2007, 22(4): 5-6
作者姓名:祁美英  王丽  于守鸿  谢辉  王梅  赵小龙  杨汉青
作者单位:青海省地方病预防控制所,西宁,811602
摘    要:目的 了解胶体金快速检测试剂盒检测鼠疫Fl抗体的应用.方法 按鼠疫胶体金快速检测试剂盒的使用说明进行,并用间接血凝法(IHA)进行比较.结果 检测了采集于青海鼠疫疫源地内及非疫源地血清共3 079份,检出阳性血清268份,对照组血清14份,结果均为阴性,与IHA结果基本一致. 结论胶体金快速检测试剂盒诊断鼠疫简便、快速、特异、敏感,最适宜现场鼠疫检测.

关 键 词:金标免疫层析法  鼠疫  诊断
文章编号:1000-3711(2007)04-0005-02
收稿时间:2006-12-19
修稿时间:2006-12-19

Comparison of Gold Immuno-chromatographic Assay and Indirect Hemagglutination Assay in Diagnosis of Plague
QI Mei-ying,WANG Li,YU Shou-hong,XIE Hui,WANG Mei,ZHAO Xiao-long,YANG Han-qing. Comparison of Gold Immuno-chromatographic Assay and Indirect Hemagglutination Assay in Diagnosis of Plague[J]. Endemic Diseases Bulletin, 2007, 22(4): 5-6
Authors:QI Mei-ying  WANG Li  YU Shou-hong  XIE Hui  WANG Mei  ZHAO Xiao-long  YANG Han-qing
Affiliation:Qinghai Institute for Endemic Disease Control and Prevention, Xining, Qinghai 811602, China
Abstract:Objective In order to comprehend the application of the Gold Immuno-chromatographic Assay(GICA) kit for detecting plague F1 antibody.Methods Plague Fl antibody was detected by using GICA kit according to the operation guide of the GICA kit,and the result was compared with that of Indirect Hemagglutination Assay(IHA)at the same time.Results Sera of 3 079 from the domestic plague natural foci and outside the epidemic areas were detected.The results showed 268 were positive and 14 in the control group were all negative by GICA and IHA.Conclusions The GICA kit for diagnosing plague was simple,rapid,specific and sensitive.It was suitable for the present detection.
Keywords:Gold Immuno-chromatographic Assay(GICA)  Plague  Diagnosis
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