牛磺酸对大鼠缺血心肌缺血再损伤的保护作用

目的探讨大鼠心肌缺血损伤与凋亡蛋白 Bcl-2和 Bax的关系及牛磺酸的影响. 方法选择健康 SD大鼠 30只,随机分为假结扎组、结扎组和牛磺酸保护组,每组 10只.结扎大鼠冠状动脉左前降支建立心肌缺血模型.检测 3组心肌线粒体中超氧化物歧化酶( superoxide dismutase,SOD)、 Ca2+-ATP酶活性和丙二醛含量,用免疫组化法检测心肌中的 Bcl-2和 Bax蛋白. 结果结扎冠状动脉左前降支可致心肌线粒体丙二醛含量升高 假结扎组 (4.89± 0.54) μ mol/g;结扎组( 9.85± 0.68) μ mol/g], SOD和 Ca2+-ATP酶活性下降 假结扎组( 8.63± 0.35) μ mol/( g· s) ,(13.97± 1.97) mmol/( g· s) ;结扎组( 6.73± 0.39) μ mol/( g· s), (8.54± 2.28) mmol/( g· s) ].缺血心肌 Bax蛋白表达呈显著升高 (t=3.931, P< 0.01),牛磺酸能明显减少缺血心肌线粒体丙二醛的生成 牛磺酸保护组 (5.46± 0.72) μ mol/g],降低心肌 Bax蛋白的表达,增加 Bcl-2蛋白的表达 ( t=3.716, P< 0.01). 结论结扎大鼠冠状动脉左前降支所致心肌缺血损伤与 Bcl-2和 Bax蛋白的表达有关 ,牛磺酸对缺血心肌 Bcl-2和 Bax蛋白的表达有较好的调控作用.

Protective effects of taurine to ischemic re-injury on ischemic myocardium in rat

AIM:To discuss the relationship between myocardium ischemia injury and apoptin Bcl-2 and Bax and the effects of taurine. METHODS:Thirty cases of healthy SD rats were selected and randomly allocated into pseudo-ligation group,ligation group and taurine protection group with 10 cases in each group.The left descending branch of coronary artery of the rat was ligated to establish myocardium ischemic model.Superoxide dismutase(SOD),Ca2+-ATPase activity and malondialdehyde (MDA) content in myocardium mitochondrion were detected in 3 groups. Apoptin Bcl-2 and Bax were detected by immunohistochemical test. RESULTS:MDA contents in mitochondrion increasedpseudo-ligation group (4.89± 0.54) μ mol/g;ligation group(9.85± 0.68) μ mol/g]and SOD and Ca2+-ATPase activity decreasedpseudo-ligation group(8.63± 0.35) μ mol/(g· s),(13.97± 1.97) mmol/(g· s);ligation group(6.73± 0.39) μ mol/(g· s),(8.54± 2.28) mmol/(g· s)]due to the ligation of the left descending branch of coronary artery.The expression of Bax apoptin in ischemic myocardium significantly increased(t=3.716,P< 0.01).Taurine could significantly decrease MDA formation in mitochondriontaurine protection group:(5.46± 0.72) μ mol/g]as well as the expression of Bax apoptin but increasing the expression of Bcl-2 apoptin(t=3.716,P< 0.01). CONCLUSION:There is a relationship between myocardium ischemic injury due to the ligation of the left descending branch of coronary artery and the expressions of apoptin Bax and Bcl-2.Taurine has a preferably regulative and controlling effect on the expression of Bax and Bcl-2 apoptins in ischemic myocardium.