靶向肿瘤血管生成的体外MR分子成像的初步研究

目的 应用GoldMagTM-CS纳米金磁微粒标记含RGD序列的环形多肽[cyclic arginine-glycineaspartic-D-phenylalanine-lysine,c(RGDfK))]构建靶向肿瘤新生血管特异性分子探针并探讨其在体内MR成像的可行性.方法 通过GoldMagTM-CS纳米金磁微粒与c...

Primary study of specific targeting of tumor angiogenesis by RGD-conjugated GoldMagTM-CS nanoparticles with a clinical 3.0 T magnetic resonance scanner in vitro

Objective To prospectively evaluate the ability of cyclic arginine-glycine-aspartic-D-phenylalanine-lysine-conjugated-GoldMagTM-CS nanoparticles to depict tumor angiogenesis with a clinical 3.0 T MR scanner system in vitro.Methods Construction of MR Specific molecular probes used GoldMagTM-CS nanoparticles and c(RGDfK)by noncovalent bond couple.The capability of synthesized probes specific targeted to HUVECs was proved by observation of flow cytometry.MR scan sequence was established and the GoldMag solution in different concentration was scanned to ascertain the lowest concentration that could be detected by MR system.The ability of RGD@GOLDMag to noninvasivesily image tumor angiogenesis was evaluated using a clinical 3.0 T MR scanner in vitro(HUVECs)divided into five groups: RGD@GOLDMag group,no RGD@GOLDMag group,competitive group,water,and gelatin group.T*2 weighted MRI was studied in Imaging Pro Plus 6.0.Unpaired student’ t test were used for statistic analysis.Results RGD@GOLDMag was successfully constructed,which could specificely target HUVECs and obviously decrease the MRI signal intensity(SI).The SI significantly decreased in group A compared with that of group B and group C(P<0.05).Conclusion RGD@GOLDMag has the potential to specifically detect and characterize tumor angiogenesis with a clinical 3.0 Tesla MR scanner in vitro.