大蒜新素对肾型高血压大鼠和老年大鼠脑皮质微粒体ATPase活力的影响

目的研究大蒜新素（ａｌｌｉｔｒｉｄｉ，Ａｌｌ）对肾型高血压大鼠和老年大鼠脑皮质微粒体ＡＴＰａｓｅ活力的影响。方法用两肾一夹法建立大鼠Ｇｏｌｄｂｌａｔｔ肾型高血压模型；以钼酸铵定磷，采用光电比色法测定ＡＴＰａｓｅ活力。结果肾型高血压大鼠和老年大鼠中，脑皮质微粒体中Ｎａ＋，Ｋ＋－ＡＴＰａｓｅ和Ｃａ２＋，Ｍｇ２＋－ＡＴＰａｓｅ活力比成年大鼠中显著降低，Ｍｇ２＋ＡＴＰａｓｅ无明显改变。大蒜新素（１５ ｍｇ·ｋｇ．ｄ－ｌ ｉｇ， ６０ ｄ）可显著提高肾型高血压大鼠和老年大鼠脑皮质微粒体中Ｎａ＋，Ｋ＋－ＡＴＰａｓｅ和Ｃａ２＋，Ｍｇ２＋－ＡＴＰａｓｅ活力，而对Ｍｇ２＋－ＡＴＰａｓｅ无明显影响。结论大蒜新素对大鼠脑皮质微粒体的Ｎａ＋，Ｋ＋－ＡＴＰａｓｅ和Ｃａ２＋，Ｍｇ２＋－ＡＴＰａｓｅ活力的提高可能有利于大蒜新素防治脑缺血作用。

Effect of allitridi on the ATPase activities of cerebral cortex microsomes in renovascular hypertensive rats and senile rats

AIM　To investigate the effect of allitridi on the ATPase activities of cerebral cortex microsomes in two-kidney one-clip renovascular hypertensive and senile rats, which are prone to the cerebral ischemia. METHODS　Cerebral cortex microsomes were purified from renohypertensive and senile Sprague-Dawley rat. ATPase activities were determined according to inorganic phosphate measurement. RESULTS　Allitridi (15 mg*kg-1 ig, 60 d) could significantly improve the Na+,K+-ATPase and Ca2+,Mg2+-ATPase activities of brain cortex microsomes in two-kidney one-clip revohypertensive rats and senile rats, while had no obvious influences on Mg2+-ATPase activity. CONCLUTION　The influences of allitridi on the ATPase activities of brain in renovascular hypertensive and senile rats are benefit to its prevention and therapy to cerebral ischemic injury.