大鼠骨髓间充质干细胞向神经细胞分化过程中Notch信号与RhoA/Rho激酶信号的协同作用 |
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引用本文: | 赵二义,王留东,文全庆,关文娟,鲁晶晶,彭涛,张博爱,贾延劼,Er-yi,Liu-dong,Quan-qing,Wen-juan,Jing-jing,Bo-ai,Yan-jie.大鼠骨髓间充质干细胞向神经细胞分化过程中Notch信号与RhoA/Rho激酶信号的协同作用[J].中原医刊,2010(9). |
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作者姓名: | 赵二义 王留东 文全庆 关文娟 鲁晶晶 彭涛 张博爱 贾延劼 Er-yi Liu-dong Quan-qing Wen-juan Jing-jing Bo-ai Yan-jie |
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基金项目: | 国家自然科学基金,河南省教育厅自然科学研究计划项目 |
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摘 要: | 目的 探讨Notch信号通路在大鼠骨髓间充质干细胞(MSCs)向神经细胞分化中与RhoA/Rho激酶信号的协同作用.方法 实验分为无血清培养基对照组和盐酸法舒地尔组.采用盐酸法舒地尔诱导大鼠MSCs分化为神经细胞.RT-PCR检测Hes1的表达变化;免疫细胞化学法检测神经元特异性烯醇化酶(NSE)、神经微丝蛋白亚单位(NF-M)和胶质纤维酸性蛋白(GFAP)的表达变化.结果 ①盐酸法舒地尔组MSCs的Hes1 mRNA转录下降(P<0.05).②盐酸法舒地尔可以诱导MSCs向神经细胞分化,NSE、NF-M的阳性表达率显著高于无血清培养基组(P<0.05).结论 盐酸法舒地尔在诱导大鼠MSCs向神经细胞分化过程中,可能存在Notch信号通路与RhoA/Rho激酶通路信号的协同作用,共同促进MSCs向神经细胞分化.
Abstract:
Objective To investigate the cross-talk between notch and Rho/Rho GTPase signaling on rat bone marrow mesenchymal stem cells differentiating into neurons. Methods The experiments were divided into serum-free medium control group and fasudil hydrochloride group. Fasudil hydrochloride induces MSCs differentiating into neurons. The expression of Hes1 mRNA was detected by RT-PCR. The expression of neuron-specific enolae(NSE), neurofilament M (NF-M) and glial fibrillary acidic protein (GFAP)was detected by immunocytochemical method. Results ①The Hes1 mRNA expressed by MSCs of fasudil hydrochloride group was significantly decreased(P<0.05).②Fasudil hydrochloride can induce MSCs differentiating into neurons and there was higher expression of neuron-specific enolae(NSE) and neurofilament-M (NF-M) than the serum-free medium control group(P<0.05).Conclusions There may be cross-talk between notch signaling and RhoA/Rho GTPase signaling on differentiation of rat bone marrow stromal cell into neurons induced by fasudil hydrochloride and they jointly promoted the differentiation of MSCs into neurons.
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关 键 词: | 大鼠 骨髓间充质干细胞 神经细胞分化 分化过程 Notch信号通路 激酶 协同作用 marrow mesenchymal stem cells GTPase notch signaling 盐酸法舒地尔 MSCs neurofilament 无血清培养基 bone marrow stromal cell 神经元特异性烯醇化酶 cross-talk 胶质纤维酸性蛋白 表达变化 NF-M |
Cross-talk between notch signaling and RhoA/Rho GTPase signaling on rat bone marrow mesenchymal stem cells differentiating into neurons |
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Abstract: | Notch Rho Fasudil hydrochloride Marrow mesenchymal stem cells Neurons |
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Keywords: | Notch Rho Fasudil hydrochloride Marrow mesenchymal stem cells Neurons |
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