CpG ODN活化外周血单个核细胞对淋巴瘤HUT-78细胞杀伤作用的研究 |
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引用本文: | 洪帆,张明智,曹玉柱,付晓瑞.CpG ODN活化外周血单个核细胞对淋巴瘤HUT-78细胞杀伤作用的研究[J].中原医刊,2010(9). |
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作者姓名: | 洪帆 张明智 曹玉柱 付晓瑞 |
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摘 要: | 目的 探讨CpG寡聚脱氧核苷酸(CpG ODN)在肿瘤免疫治疗中的作用.方法 将CpG ODN刺激体外培养的人外周血单个核细胞(PBMC),ELISA法检测其分泌细胞因子白细胞介素-12(IL-12)、干扰素-γ(INF-γ)的表达, MTT法检测CpG ODN作用后的PBMC对淋巴瘤细胞株HUT-78细胞杀伤活性.结果 与对照组相比,经CpG ODN作用后的PBMC对HUT-78细胞杀伤活性显著提高(P<0.05),同时CpG ODN能明显促进PBMC分泌细胞因子IL-12、INF-γ(P<0.05).结论 CpG ODN可增强PBMC介导的抗淋巴瘤细胞的作用,其杀伤活性与分泌细胞因子有关.
Abstract:
Objective To study the immunotherapy role of CpG ODN(CpGoligodeoxynucleotide) on tumor.Methods After treated with synthetic CpG ODN for 48 hours, the inhibiting effect of PBMC on HUT-78 cells were detected by MTT method.The secretions of TNF-γ and IL-12 from PBMC determined using ELISA method.Results The PBMC treated with CpG ODN enhanced their antitumor effect on HUT-78 cells and increased the secretion levels of TNF-γ and IL-12.Conclusions CpG ODN increases the cytotoxicity of PBMC on HUT-78 cells in vitro, as well as facilitates the IL-12 and TNF-γ secretion.
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关 键 词: | CpG ODN 活化 人外周血单个核细胞 淋巴瘤细胞 细胞杀伤作用 cells PBMC 分泌细胞因子 IL-12 细胞杀伤活性 细胞因子白细胞介素 inhibiting effect CpG寡聚脱氧核苷酸 antitumor effect treated 肿瘤免疫治疗 INF-γ ELISA法检测 体外培养 瘤细胞株 |
Study of killing activity of PBMC stimulated by CpG ODN on HUT-78 cells |
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Abstract: | CpG ODN PBMC Immunotherapy |
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Keywords: | CpG ODN PBMC Immunotherapy |
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